Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 644 | 3' | -59.9 | AC_000017.1 | + | 10351 | 0.66 | 0.380851 |
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Target: 5'- ----gUGCGGCGgcgGCUGGcgguagaGGGGCc -3' miRNA: 3'- uguaaGCGCCGCa--CGACCugg----CCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 16404 | 0.67 | 0.330174 |
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Target: 5'- gGCugcUGCGGCG-GCcgcucgucgccUGGACCuggGGGGCa -3' miRNA: 3'- -UGuaaGCGCCGCaCG-----------ACCUGG---CCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 10751 | 0.67 | 0.299247 |
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Target: 5'- cGCAUggaUCaCGGCGgacgGCcGGAUaCGGGGCu -3' miRNA: 3'- -UGUA---AGcGCCGCa---CGaCCUG-GCCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 16273 | 0.68 | 0.284644 |
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Target: 5'- cGCGUUgGgCGGCaGUGCcGGGUCGGcGGCg -3' miRNA: 3'- -UGUAAgC-GCCG-CACGaCCUGGCC-CCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 65 | 0.68 | 0.25061 |
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Target: 5'- gACGUggcgCGgGGCGUG--GGAaCGGGGCg -3' miRNA: 3'- -UGUAa---GCgCCGCACgaCCUgGCCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 1637 | 0.68 | 0.25061 |
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Target: 5'- ---cUUGCauGGCGUGUuaaaUGGGgCGGGGCu -3' miRNA: 3'- uguaAGCG--CCGCACG----ACCUgGCCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 35937 | 0.68 | 0.25061 |
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Target: 5'- gACGUggcgCGgGGCGUG--GGAaCGGGGCg -3' miRNA: 3'- -UGUAa---GCgCCGCACgaCCUgGCCCCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 18262 | 0.69 | 0.243588 |
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Target: 5'- gGCAUUaGCGGgGUGgUGGACCuGGccaaccaGGCa -3' miRNA: 3'- -UGUAAgCGCCgCACgACCUGG-CC-------CCG- -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 8533 | 0.7 | 0.208681 |
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Target: 5'- uACGguaccgCGCGGCGgGCggUGGGCCGcGGGg -3' miRNA: 3'- -UGUaa----GCGCCGCaCG--ACCUGGC-CCCg -5' |
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| 644 | 3' | -59.9 | AC_000017.1 | + | 19145 | 1.1 | 0.00018 |
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Target: 5'- gACAUUCGCGGCGUGCUGGACCGGGGCc -3' miRNA: 3'- -UGUAAGCGCCGCACGACCUGGCCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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