Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 644 | 5' | -58 | AC_000017.1 | + | 16211 | 0.66 | 0.377666 |
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Target: 5'- -cGCCg-AC-CCGGCACUGCCgccCAAc -3' miRNA: 3'- uuCGGgaUGaGGCCGUGACGGau-GUU- -5' |
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| 644 | 5' | -58 | AC_000017.1 | + | 914 | 0.67 | 0.292301 |
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Target: 5'- aAGGCUCUcuGCUCCGGCugcucggGCUGCCg---- -3' miRNA: 3'- -UUCGGGA--UGAGGCCG-------UGACGGauguu -5' |
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| 644 | 5' | -58 | AC_000017.1 | + | 19678 | 0.7 | 0.200502 |
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Target: 5'- -uGCCCc-CUCUGGCACUGCCa---- -3' miRNA: 3'- uuCGGGauGAGGCCGUGACGGauguu -5' |
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| 644 | 5' | -58 | AC_000017.1 | + | 12239 | 0.7 | 0.19497 |
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Target: 5'- cAGCCCaGgUCCGGCcccaGCUGCCUcCAGg -3' miRNA: 3'- uUCGGGaUgAGGCCG----UGACGGAuGUU- -5' |
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| 644 | 5' | -58 | AC_000017.1 | + | 12120 | 0.71 | 0.164516 |
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Target: 5'- cGAGUCCUACUUUgacgcgGGCGCUGaCCUGCGc -3' miRNA: 3'- -UUCGGGAUGAGG------CCGUGAC-GGAUGUu -5' |
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| 644 | 5' | -58 | AC_000017.1 | + | 19180 | 1.03 | 0.000549 |
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Target: 5'- uAAGCCCUACUCCGGCACUGCCUACAAc -3' miRNA: 3'- -UUCGGGAUGAGGCCGUGACGGAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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