Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6496 | 5' | -50.5 | NC_001847.1 | + | 16016 | 1.11 | 0.007109 |
Target: 5'- cUCGGUGUACUCCAUGUAGUACAGCGGc -3' miRNA: 3'- -AGCCACAUGAGGUACAUCAUGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 52600 | 0.7 | 0.944996 |
Target: 5'- gCGGUGcGCUUCG-GUAGUGCGGuCGa -3' miRNA: 3'- aGCCACaUGAGGUaCAUCAUGUC-GCc -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 24580 | 0.69 | 0.961441 |
Target: 5'- cCGGccGUG-UCCAUGUGGUaAUGGCGGu -3' miRNA: 3'- aGCCa-CAUgAGGUACAUCA-UGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 30909 | 0.69 | 0.968228 |
Target: 5'- cCGGUGagGCgcgcgCCGgc-GGUGCGGCGGa -3' miRNA: 3'- aGCCACa-UGa----GGUacaUCAUGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 96620 | 0.68 | 0.97412 |
Target: 5'- aCGGUGUACggCCAg--AGcACAGCGc -3' miRNA: 3'- aGCCACAUGa-GGUacaUCaUGUCGCc -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 18104 | 0.68 | 0.979175 |
Target: 5'- aCGGgGUGCUCCAgc-AGcuccGCGGCGGu -3' miRNA: 3'- aGCCaCAUGAGGUacaUCa---UGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 42323 | 0.67 | 0.982862 |
Target: 5'- gCGaGUGUuucgugcugugggcGC-CCGUGUGGcGCGGCGGc -3' miRNA: 3'- aGC-CACA--------------UGaGGUACAUCaUGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 7214 | 0.67 | 0.983458 |
Target: 5'- gCGGcUGgGCcgUCCAUGgcGcUGCAGCGGc -3' miRNA: 3'- aGCC-ACaUG--AGGUACauC-AUGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 90992 | 0.67 | 0.989166 |
Target: 5'- -gGGUGUGCUCCAgcgAgacgagagccacgccGUGcCGGCGGa -3' miRNA: 3'- agCCACAUGAGGUacaU---------------CAU-GUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 17318 | 0.66 | 0.992388 |
Target: 5'- cCGGUccGCUCU-UGUGGUGCAG-GGc -3' miRNA: 3'- aGCCAcaUGAGGuACAUCAUGUCgCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 120169 | 0.66 | 0.993402 |
Target: 5'- gCGGUGccuguUGCUCUGgaugGUGGUGCuggccgcgcgAGCGGc -3' miRNA: 3'- aGCCAC-----AUGAGGUa---CAUCAUG----------UCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 3068 | 0.66 | 0.993402 |
Target: 5'- -aGGuUGUGCUC---GUAGUGCAGCa- -3' miRNA: 3'- agCC-ACAUGAGguaCAUCAUGUCGcc -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 116667 | 0.66 | 0.993402 |
Target: 5'- cUGGUGgcCUgCGUGUGGguccgGCGcuGCGGg -3' miRNA: 3'- aGCCACauGAgGUACAUCa----UGU--CGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 102524 | 0.66 | 0.993402 |
Target: 5'- aCGGcGUcgugcagccGCUCCGUGUcGgccgcgACGGCGGc -3' miRNA: 3'- aGCCaCA---------UGAGGUACAuCa-----UGUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 122269 | 0.66 | 0.994045 |
Target: 5'- cCGGguccaccGUGCUCCAcGUGGUuauuaggagcccauGCGGCGu -3' miRNA: 3'- aGCCa------CAUGAGGUaCAUCA--------------UGUCGCc -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 72474 | 0.66 | 0.995105 |
Target: 5'- aCGGcGUGCUgaUgAUGgcGUAcCAGCGGa -3' miRNA: 3'- aGCCaCAUGA--GgUACauCAU-GUCGCC- -5' |
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6496 | 5' | -50.5 | NC_001847.1 | + | 6266 | 0.66 | 0.995105 |
Target: 5'- aCGGUcGUGgUCgAUGUAcucgcgcagGCAGCGGa -3' miRNA: 3'- aGCCA-CAUgAGgUACAUca-------UGUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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