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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 650 | 3' | -48.9 | AC_000017.1 | + | 12895 | 0.66 | 0.893677 |
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Target: 5'- aAGGCCUGCAGAccGUaaaccugagccaggcUUUCAAgaacuugcaggggcUGUGGGGg -3' miRNA: 3'- -UCCGGACGUUU--UA---------------AAGGUU--------------GCACCUC- -5' |
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| 650 | 3' | -48.9 | AC_000017.1 | + | 350 | 0.67 | 0.87667 |
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Target: 5'- aGGGCC-GCGGGGacUUUgaCCGuuuACGUGGAGa -3' miRNA: 3'- -UCCGGaCGUUUU--AAA--GGU---UGCACCUC- -5' |
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| 650 | 3' | -48.9 | AC_000017.1 | + | 4153 | 0.67 | 0.868103 |
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Target: 5'- aGGGuCCUGUGuauuuUUUCCAggACGUGGuAGa -3' miRNA: 3'- -UCC-GGACGUuuu--AAAGGU--UGCACC-UC- -5' |
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| 650 | 3' | -48.9 | AC_000017.1 | + | 9153 | 0.71 | 0.651821 |
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Target: 5'- cAGcGCCUGCGAAAcuacgCCGucuucgcccggcACGUGGAGc -3' miRNA: 3'- -UC-CGGACGUUUUaaa--GGU------------UGCACCUC- -5' |
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| 650 | 3' | -48.9 | AC_000017.1 | + | 25212 | 1.09 | 0.002443 |
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Target: 5'- cAGGCCUGCAAAAUUUCCAACGUGGAGc -3' miRNA: 3'- -UCCGGACGUUUUAAAGGUUGCACCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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