Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 651 | 3' | -61.9 | AC_000017.1 | + | 16426 | 0.66 | 0.2342 |
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Target: 5'- cUCGGuUAGCGGCCuGCGcGuGCCCgUGCg- -3' miRNA: 3'- -AGUC-GUCGUCGG-CGC-C-CGGGaACGaa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 26600 | 0.66 | 0.222068 |
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Target: 5'- aCGGCGGCGGCUGCuuggacuuaccGGCCCUgGUUc -3' miRNA: 3'- aGUCGUCGUCGGCGc----------CCGGGAaCGAa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 16980 | 0.66 | 0.216205 |
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Target: 5'- gCAGCAGguGCUGCccGCgCUUGCa- -3' miRNA: 3'- aGUCGUCguCGGCGccCGgGAACGaa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 12449 | 0.67 | 0.183758 |
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Target: 5'- cCGGCAGCAGCCaCaGGCCaaccgGCUc -3' miRNA: 3'- aGUCGUCGUCGGcGcCCGGgaa--CGAa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 31611 | 0.67 | 0.183758 |
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Target: 5'- aUCAGUGGCAGaaaGgGGGCCCgaUGUUUg -3' miRNA: 3'- -AGUCGUCGUCgg-CgCCCGGGa-ACGAA- -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 11809 | 0.68 | 0.169203 |
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Target: 5'- gCAGcCAGCGGCCcuCGGGCUCUacuaUGUUUa -3' miRNA: 3'- aGUC-GUCGUCGGc-GCCCGGGA----ACGAA- -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 22181 | 0.69 | 0.134945 |
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Target: 5'- gCAGCagaauaggccacaGGCGGCCGaguuGGGCCCcUGCa- -3' miRNA: 3'- aGUCG-------------UCGUCGGCg---CCCGGGaACGaa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 23242 | 0.7 | 0.125731 |
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Target: 5'- gCAGCGgugcagccacaacgcGCAGcCCGUGGGCUCguggUGCUUg -3' miRNA: 3'- aGUCGU---------------CGUC-GGCGCCCGGGa---ACGAA- -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 16360 | 0.72 | 0.083127 |
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Target: 5'- --cGCAGCAGCCGCGG-CCauuagUGCUa -3' miRNA: 3'- aguCGUCGUCGGCGCCcGGga---ACGAa -5' |
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| 651 | 3' | -61.9 | AC_000017.1 | + | 26193 | 1.05 | 0.000177 |
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Target: 5'- aUCAGCAGCAGCCGCGGGCCCUUGCUUc -3' miRNA: 3'- -AGUCGUCGUCGGCGCCCGGGAACGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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