Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 652 | 3' | -57.9 | AC_000017.1 | + | 9553 | 0.67 | 0.412646 |
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Target: 5'- cGCCGucaccGAGaCCAuGC-GCCGUCGCCg -3' miRNA: 3'- -CGGCca---UUCaGGUuCGuCGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 10418 | 0.69 | 0.279525 |
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Target: 5'- cGCUGGccccUCUAccgcCAGCCGCCGCCg -3' miRNA: 3'- -CGGCCauucAGGUuc--GUCGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 11558 | 0.7 | 0.272448 |
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Target: 5'- -gCGGUua--CCAGGuCGGCgGCCGCCa -3' miRNA: 3'- cgGCCAuucaGGUUC-GUCGgCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 12699 | 0.7 | 0.272448 |
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Target: 5'- aGCCGGUccAGGUUgGucuGCAcGuuGCCGCUg -3' miRNA: 3'- -CGGCCA--UUCAGgUu--CGU-CggCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 10870 | 0.72 | 0.202222 |
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Target: 5'- cGCgCGGccAGUggCCAaaaaaagcuagcgcAGCAGCCGCCGCg -3' miRNA: 3'- -CG-GCCauUCA--GGU--------------UCGUCGGCGGCGg -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 2275 | 0.74 | 0.138628 |
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Target: 5'- gGCCGGcucucGGGuUCCAugGGCucugcuccuGCCGCCGCCg -3' miRNA: 3'- -CGGCCa----UUC-AGGU--UCGu--------CGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 12510 | 0.74 | 0.127397 |
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Target: 5'- aGCCGGUuGG-CCu-GUGGCUGCUGCCg -3' miRNA: 3'- -CGGCCAuUCaGGuuCGUCGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 16312 | 0.79 | 0.063777 |
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Target: 5'- gGCCGGUGcgacgugcgcGGUU-AAGCagGGCCGCCGCCg -3' miRNA: 3'- -CGGCCAU----------UCAGgUUCG--UCGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 31333 | 0.8 | 0.050412 |
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Target: 5'- uGCCGGccuuGUCCAGGgcuaGGCCGCUGCCc -3' miRNA: 3'- -CGGCCauu-CAGGUUCg---UCGGCGGCGG- -5' |
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| 652 | 3' | -57.9 | AC_000017.1 | + | 12410 | 0.66 | 0.461563 |
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Target: 5'- gGCCGGacGGcuggCUcuGCAG-CGCCGCCc -3' miRNA: 3'- -CGGCCauUCa---GGuuCGUCgGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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