Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
652 | 5' | -52.6 | AC_000017.1 | + | 11094 | 0.74 | 0.268365 |
Target: 5'- aAGAGCAAgAGCAGCGgCAgacaugcaGGGC-ACCc -3' miRNA: 3'- -UCUCGUUgUUGUCGCgGU--------UCCGaUGG- -5' |
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652 | 5' | -52.6 | AC_000017.1 | + | 26765 | 0.79 | 0.131592 |
Target: 5'- cGGcAGCAACAGCAGCgGCCAcacagaagcaaAGGCgACCg -3' miRNA: 3'- -UC-UCGUUGUUGUCG-CGGU-----------UCCGaUGG- -5' |
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652 | 5' | -52.6 | AC_000017.1 | + | 26572 | 1.13 | 0.00046 |
Target: 5'- aAGAGCAACAACAGCGCCAAGGCUACCg -3' miRNA: 3'- -UCUCGUUGUUGUCGCGGUUCCGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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