Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 656 | 3' | -57 | AC_000017.1 | + | 27137 | 0.66 | 0.497181 |
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Target: 5'- gCGAAa-ACUaCGUCAUCUCCaGCGGCc -3' miRNA: 3'- -GCUUggUGA-GCAGUGGAGGgUGCCGu -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 16291 | 0.66 | 0.497181 |
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Target: 5'- gCGAGCCGCUCGaagGCUggCCGCGGg- -3' miRNA: 3'- -GCUUGGUGAGCag-UGGagGGUGCCgu -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 16338 | 0.66 | 0.497181 |
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Target: 5'- uCGAGCgGCUCGcaUgGCCgCCCGuCGGCc -3' miRNA: 3'- -GCUUGgUGAGC--AgUGGaGGGU-GCCGu -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 829 | 0.68 | 0.37982 |
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Target: 5'- gGAGCCGC-C-UCACCUUUCcCGGCAg -3' miRNA: 3'- gCUUGGUGaGcAGUGGAGGGuGCCGU- -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 9462 | 0.73 | 0.195152 |
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Target: 5'- uCGAcaaAgCGCUCGaUCAUCUcCCCGCGGCGa -3' miRNA: 3'- -GCU---UgGUGAGC-AGUGGA-GGGUGCCGU- -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 16397 | 0.74 | 0.152303 |
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Target: 5'- gCGGcgGCCGCUCGUCGCCUggaCCugGGgGg -3' miRNA: 3'- -GCU--UGGUGAGCAGUGGAg--GGugCCgU- -5' |
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| 656 | 3' | -57 | AC_000017.1 | + | 31224 | 1.08 | 0.000459 |
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Target: 5'- cCGAACCACUCGUCACCUCCCACGGCAu -3' miRNA: 3'- -GCUUGGUGAGCAGUGGAGGGUGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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