Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 656 | 5' | -56.2 | AC_000017.1 | + | 18263 | 0.66 | 0.519278 |
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Target: 5'- -gCAuuAGcgGGGUGGUGGaCCUGGCCa- -3' miRNA: 3'- gaGU--UUuaCCCGUCGCC-GGAUCGGga -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 22178 | 0.67 | 0.415048 |
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Target: 5'- -gCAGAAUaGGCcacaGGCGGCCgaguugGGCCCc -3' miRNA: 3'- gaGUUUUAcCCG----UCGCCGGa-----UCGGGa -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 24174 | 0.67 | 0.405337 |
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Target: 5'- aCUCAGAggGGGCGGUuaGGCU--GUCCUc -3' miRNA: 3'- -GAGUUUuaCCCGUCG--CCGGauCGGGA- -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 18234 | 0.68 | 0.386351 |
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Target: 5'- -aCAAAagGuGGUAGaUGGCCUGGCCUc -3' miRNA: 3'- gaGUUUuaC-CCGUC-GCCGGAUCGGGa -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 2190 | 0.69 | 0.308453 |
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Target: 5'- -cCAGGcgGcGGCGGCGGCaggagcagAGCCCa -3' miRNA: 3'- gaGUUUuaC-CCGUCGCCGga------UCGGGa -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 4633 | 0.7 | 0.263527 |
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Target: 5'- -------cGGGCGGCGGCCUGGgCg- -3' miRNA: 3'- gaguuuuaCCCGUCGCCGGAUCgGga -5' |
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| 656 | 5' | -56.2 | AC_000017.1 | + | 31258 | 1.08 | 0.000424 |
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Target: 5'- gCUCAAAAUGGGCAGCGGCCUAGCCCUg -3' miRNA: 3'- -GAGUUUUACCCGUCGCCGGAUCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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