Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6566 | 5' | -56.4 | NC_001847.1 | + | 73134 | 0.68 | 0.838048 |
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Target: 5'- gCCGUGcUCgcGGACCUGGAgG-AGGcGGc -3' miRNA: 3'- -GGCACaAGa-CCUGGGCCUgCaUCC-CC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 10527 | 0.68 | 0.838048 |
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Target: 5'- aCGUGUUCUuc-CCCGGGCcc-GGGGa -3' miRNA: 3'- gGCACAAGAccuGGGCCUGcauCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 88246 | 0.68 | 0.813151 |
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Target: 5'- gCCGgcgcgGcgCgGGGCgCCGGGCGgggacgGGGGGa -3' miRNA: 3'- -GGCa----CaaGaCCUG-GGCCUGCa-----UCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 32072 | 0.72 | 0.621779 |
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Target: 5'- cCCGggcucgGgcccCUGGGCgCCGGGCGUcGGGGc -3' miRNA: 3'- -GGCa-----Caa--GACCUG-GGCCUGCAuCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 28647 | 0.72 | 0.621779 |
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Target: 5'- gCCGUGUUC--GugCCGGAgaUGGGGGa -3' miRNA: 3'- -GGCACAAGacCugGGCCUgcAUCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 15304 | 0.72 | 0.601748 |
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Target: 5'- aCCGgcacGgcgUCggGGGCCgCGGGCGUAGcGGGg -3' miRNA: 3'- -GGCa---Ca--AGa-CCUGG-GCCUGCAUC-CCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 107192 | 0.72 | 0.591761 |
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Target: 5'- gCGag--CUGGGCCCGGACGcgAGGaGGa -3' miRNA: 3'- gGCacaaGACCUGGGCCUGCa-UCC-CC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 2232 | 0.66 | 0.919958 |
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Target: 5'- gCCGcgGcgCUGGGCgCGGGCGUGuGGu -3' miRNA: 3'- -GGCa-CaaGACCUGgGCCUGCAUcCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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