Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6566 | 5' | -56.4 | NC_001847.1 | + | 62781 | 0.71 | 0.64184 |
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Target: 5'- aCCGgg-UCUGGGCCCGG----GGGGGc -3' miRNA: 3'- -GGCacaAGACCUGGGCCugcaUCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 28647 | 0.72 | 0.621779 |
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Target: 5'- gCCGUGUUC--GugCCGGAgaUGGGGGa -3' miRNA: 3'- -GGCACAAGacCugGGCCUgcAUCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 32072 | 0.72 | 0.621779 |
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Target: 5'- cCCGggcucgGgcccCUGGGCgCCGGGCGUcGGGGc -3' miRNA: 3'- -GGCa-----Caa--GACCUG-GGCCUGCAuCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 134885 | 0.72 | 0.621778 |
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Target: 5'- cCCGggcucgGgcccCUGGGCgCCGGGCGUcGGGGc -3' miRNA: 3'- -GGCa-----Caa--GACCUG-GGCCUGCAuCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 131460 | 0.72 | 0.621778 |
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Target: 5'- gCCGUGUUC--GugCCGGAgaUGGGGGa -3' miRNA: 3'- -GGCACAAGacCugGGCCUgcAUCCCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 15304 | 0.72 | 0.601748 |
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Target: 5'- aCCGgcacGgcgUCggGGGCCgCGGGCGUAGcGGGg -3' miRNA: 3'- -GGCa---Ca--AGa-CCUGG-GCCUGCAUC-CCC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 107192 | 0.72 | 0.591761 |
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Target: 5'- gCGag--CUGGGCCCGGACGcgAGGaGGa -3' miRNA: 3'- gGCacaaGACCUGGGCCUGCa-UCC-CC- -5' |
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| 6566 | 5' | -56.4 | NC_001847.1 | + | 41692 | 1.12 | 0.002102 |
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Target: 5'- cCCGUGUUCUGGACCCGGACGUAGGGGu -3' miRNA: 3'- -GGCACAAGACCUGGGCCUGCAUCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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