Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 657 | 3' | -49.3 | AC_000017.1 | + | 30478 | 0.66 | 0.920018 |
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Target: 5'- aUCUCAgugggAGUGggAGAAGGAGggcgaugcugauuGGCUGGg -3' miRNA: 3'- -GGAGUg----UCACagUCUUCCUU-------------UCGACC- -5' |
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| 657 | 3' | -49.3 | AC_000017.1 | + | 29555 | 0.66 | 0.907171 |
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Target: 5'- gUUCACuGUGUCGaccaugucGAAGGcgGGCUGc -3' miRNA: 3'- gGAGUGuCACAGU--------CUUCCuuUCGACc -5' |
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| 657 | 3' | -49.3 | AC_000017.1 | + | 601 | 0.66 | 0.899957 |
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Target: 5'- gUCUCAuuuucaguccCGGUGUCGGAgcggcucggAGGAGAaaacucuacucGCUGGc -3' miRNA: 3'- -GGAGU----------GUCACAGUCU---------UCCUUU-----------CGACC- -5' |
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| 657 | 3' | -49.3 | AC_000017.1 | + | 18613 | 0.73 | 0.551124 |
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Target: 5'- uUCUgCugGGUGUCAGcgGGGGGAGGCaGGu -3' miRNA: 3'- -GGA-GugUCACAGUC--UUCCUUUCGaCC- -5' |
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| 657 | 3' | -49.3 | AC_000017.1 | + | 31515 | 1.13 | 0.00142 |
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Target: 5'- cCCUCACAGUGUCAGAAGGAAAGCUGGc -3' miRNA: 3'- -GGAGUGUCACAGUCUUCCUUUCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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