Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 661 | 3' | -49.2 | AC_000018.1 | + | 18615 | 0.66 | 0.916324 |
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Target: 5'- --gAGGUCGCgCGCCGAGgCuGAGUUg -3' miRNA: 3'- cgaUCUAGUG-GUGGCUUgGuCUUAGa -5' |
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| 661 | 3' | -49.2 | AC_000018.1 | + | 25683 | 0.66 | 0.909472 |
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Target: 5'- gGCgagGGGUCuuCuCCGGGgCAGAGUCUg -3' miRNA: 3'- -CGa--UCUAGugGuGGCUUgGUCUUAGA- -5' |
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| 661 | 3' | -49.2 | AC_000018.1 | + | 2066 | 0.66 | 0.887034 |
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Target: 5'- cCUAGAggCugCACUG-GCCAGuAAUCUa -3' miRNA: 3'- cGAUCUa-GugGUGGCuUGGUC-UUAGA- -5' |
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| 661 | 3' | -49.2 | AC_000018.1 | + | 22294 | 0.7 | 0.727987 |
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Target: 5'- --gGGAUCACCaACUuggGAACUGGAAUCUc -3' miRNA: 3'- cgaUCUAGUGG-UGG---CUUGGUCUUAGA- -5' |
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| 661 | 3' | -49.2 | AC_000018.1 | + | 6929 | 0.73 | 0.522022 |
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Target: 5'- cCUGGAuguacucgUCACCGCCGAGCUgguagccaagcugcaAGAGUCa -3' miRNA: 3'- cGAUCU--------AGUGGUGGCUUGG---------------UCUUAGa -5' |
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| 661 | 3' | -49.2 | AC_000018.1 | + | 1746 | 1.1 | 0.001995 |
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Target: 5'- aGCUAGAUCACCACCGAACCAGAAUCUc -3' miRNA: 3'- -CGAUCUAGUGGUGGCUUGGUCUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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