Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 665 | 3' | -57.2 | AC_000018.1 | + | 2567 | 0.67 | 0.434283 |
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Target: 5'- gGCcAGGGgUUGUCGGCAUGGaaGCa -3' miRNA: 3'- gCGcUCCCgAGCAGUCGUACUggUGg -5' |
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| 665 | 3' | -57.2 | AC_000018.1 | + | 28225 | 0.68 | 0.405536 |
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Target: 5'- cCGCuaccacccAGGGgUCGUCAGUAgggaGACCGCg -3' miRNA: 3'- -GCGc-------UCCCgAGCAGUCGUa---CUGGUGg -5' |
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| 665 | 3' | -57.2 | AC_000018.1 | + | 24860 | 0.68 | 0.378016 |
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Target: 5'- aGCGAGauagcuGCUCGUCA-C-UGACUACCg -3' miRNA: 3'- gCGCUCc-----CGAGCAGUcGuACUGGUGG- -5' |
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| 665 | 3' | -57.2 | AC_000018.1 | + | 18267 | 0.68 | 0.369126 |
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Target: 5'- --aGGGGGCUCGUCugcgcGCGUcACCAgCg -3' miRNA: 3'- gcgCUCCCGAGCAGu----CGUAcUGGUgG- -5' |
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| 665 | 3' | -57.2 | AC_000018.1 | + | 16316 | 0.69 | 0.343326 |
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Target: 5'- uCGCGGuuGGGCcaUGUUGGCaAUGGCCGCUg -3' miRNA: 3'- -GCGCU--CCCGa-GCAGUCG-UACUGGUGG- -5' |
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| 665 | 3' | -57.2 | AC_000018.1 | + | 8160 | 1.12 | 0.000213 |
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Target: 5'- cCGCGAGGGCUCGUCAGCAUGACCACCa -3' miRNA: 3'- -GCGCUCCCGAGCAGUCGUACUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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