Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6650 | 5' | -50.8 | NC_001847.1 | + | 64935 | 0.66 | 0.989694 |
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Target: 5'- -gCCGGcGGGGCGGGUGgcc-GCUUUUg -3' miRNA: 3'- caGGCU-CCUCGCUCACaucaUGAAAG- -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 36134 | 0.66 | 0.989694 |
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Target: 5'- -cCCGAGGcAGCGc-UGUGGUACg--- -3' miRNA: 3'- caGGCUCC-UCGCucACAUCAUGaaag -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 114351 | 0.66 | 0.98664 |
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Target: 5'- -gUCGGGGAGcCGAGUaaAGUACUggcUCa -3' miRNA: 3'- caGGCUCCUC-GCUCAcaUCAUGAa--AG- -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 116356 | 0.67 | 0.973267 |
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Target: 5'- --aCGGGGAGCuGGUGUgcguGGUGCUggCg -3' miRNA: 3'- cagGCUCCUCGcUCACA----UCAUGAaaG- -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 41625 | 0.69 | 0.943147 |
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Target: 5'- cUCCGGGGccgccgGGCGAGUG-GGUGCg--- -3' miRNA: 3'- cAGGCUCC------UCGCUCACaUCAUGaaag -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 108933 | 0.72 | 0.842801 |
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Target: 5'- -aCCGGGGAcUGGGUGgAGUGCUUUUa -3' miRNA: 3'- caGGCUCCUcGCUCACaUCAUGAAAG- -5' |
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| 6650 | 5' | -50.8 | NC_001847.1 | + | 97322 | 1.11 | 0.005364 |
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Target: 5'- cGUCCGAGGAGCGAGUGUAGUACUUUCg -3' miRNA: 3'- -CAGGCUCCUCGCUCACAUCAUGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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