Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6656 | 3' | -61.4 | NC_001847.1 | + | 80569 | 0.67 | 0.62109 |
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Target: 5'- cGCCg-CCCagCCCGCGGGGccGUCgUCCUCc -3' miRNA: 3'- -CGGgaGGG--GGGUGUCCC--UAG-AGGAGc -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 92141 | 0.67 | 0.62109 |
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Target: 5'- gGCCCggcgcgCCCCCC-CGGcGGcgUUCC-CGg -3' miRNA: 3'- -CGGGa-----GGGGGGuGUC-CCuaGAGGaGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 15422 | 0.67 | 0.610223 |
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Target: 5'- cGCCCUCggcgCCUCCGuuggcuuCGGGGGUCUgacUCUCGc -3' miRNA: 3'- -CGGGAG----GGGGGU-------GUCCCUAGA---GGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 126525 | 0.67 | 0.581687 |
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Target: 5'- gGCCgCUCCCCCgGCGGGGcUUgcgCUgCGg -3' miRNA: 3'- -CGG-GAGGGGGgUGUCCCuAGa--GGaGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 25663 | 0.68 | 0.562163 |
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Target: 5'- uCCCUCCCCCCGCuAGcacaggcuugaaGGAacacugugUUCCUCGc -3' miRNA: 3'- cGGGAGGGGGGUG-UC------------CCUa-------GAGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 78104 | 0.68 | 0.552466 |
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Target: 5'- -gCCUCggCCCACAGGGcgcgCUCCUUGu -3' miRNA: 3'- cgGGAGggGGGUGUCCCua--GAGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 32354 | 0.68 | 0.533231 |
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Target: 5'- gGCCCagCCCCCgCGCGGGGGggcgCCggCGu -3' miRNA: 3'- -CGGGa-GGGGG-GUGUCCCUaga-GGa-GC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 29902 | 0.71 | 0.366945 |
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Target: 5'- gGCCCUgCCggCCgCCGCGGGGggCcCCUCGg -3' miRNA: 3'- -CGGGA-GG--GG-GGUGUCCCuaGaGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 6202 | 0.66 | 0.680277 |
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Target: 5'- aCCCcCUCCCCAUuGGGA-CUCCcCGc -3' miRNA: 3'- cGGGaGGGGGGUGuCCCUaGAGGaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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