Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6656 | 3' | -61.4 | NC_001847.1 | + | 32354 | 0.68 | 0.533231 |
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Target: 5'- gGCCCagCCCCCgCGCGGGGGggcgCCggCGu -3' miRNA: 3'- -CGGGa-GGGGG-GUGUCCCUaga-GGa-GC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 68507 | 0.68 | 0.523703 |
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Target: 5'- aGUCCacaaacgCgCCCCCGCcgucGGGAUCgUCCUCGc -3' miRNA: 3'- -CGGGa------G-GGGGGUGu---CCCUAG-AGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 132715 | 0.69 | 0.477136 |
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Target: 5'- gGCCCUgCCggCCgCCGCGGGGGcC-CCUCGg -3' miRNA: 3'- -CGGGA-GG--GG-GGUGUCCCUaGaGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 66688 | 0.71 | 0.398962 |
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Target: 5'- aCCCUCgCCUCCACAgcGGGcgCUCC-CGc -3' miRNA: 3'- cGGGAG-GGGGGUGU--CCCuaGAGGaGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 29902 | 0.71 | 0.366945 |
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Target: 5'- gGCCCUgCCggCCgCCGCGGGGggCcCCUCGg -3' miRNA: 3'- -CGGGA-GG--GG-GGUGUCCCuaGaGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 117344 | 0.73 | 0.273062 |
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Target: 5'- cGCCCUCCCgCCCGCuGGGGcccgUCcugaaccuagcggCCUCGg -3' miRNA: 3'- -CGGGAGGG-GGGUGuCCCU----AGa------------GGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 47205 | 0.75 | 0.21325 |
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Target: 5'- aCCUUCCgCuCCCGCAGGGc-CUCCUCGg -3' miRNA: 3'- cGGGAGG-G-GGGUGUCCCuaGAGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 93457 | 0.77 | 0.171639 |
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Target: 5'- aGCCCagcUCCCCCaGCA-GGAUCUCCUUGa -3' miRNA: 3'- -CGGG---AGGGGGgUGUcCCUAGAGGAGC- -5' |
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| 6656 | 3' | -61.4 | NC_001847.1 | + | 102369 | 1.1 | 0.000761 |
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Target: 5'- gGCCCUCCCCCCACAGGGAUCUCCUCGa -3' miRNA: 3'- -CGGGAGGGGGGUGUCCCUAGAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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