Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 666 | 3' | -55.4 | AC_000018.1 | + | 9433 | 0.66 | 0.569091 |
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Target: 5'- --uUCAGgGAGAUG-CGCgGAGGCGu -3' miRNA: 3'- uacAGUCgCUCUACgGCGaCUCUGCu -5' |
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| 666 | 3' | -55.4 | AC_000018.1 | + | 27155 | 0.67 | 0.491607 |
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Target: 5'- gGUGUUGGCGGGGUGUgGUggUGAGAgcUGAc -3' miRNA: 3'- -UACAGUCGCUCUACGgCG--ACUCU--GCU- -5' |
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| 666 | 3' | -55.4 | AC_000018.1 | + | 17550 | 0.67 | 0.480919 |
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Target: 5'- gAUGUUggGGCGAGGgaugcGCCGCcacAGACGAa -3' miRNA: 3'- -UACAG--UCGCUCUa----CGGCGac-UCUGCU- -5' |
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| 666 | 3' | -55.4 | AC_000018.1 | + | 31415 | 0.7 | 0.34519 |
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Target: 5'- -aGggaAGUGAGGUGCUGCUGGGA-GAa -3' miRNA: 3'- uaCag-UCGCUCUACGGCGACUCUgCU- -5' |
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| 666 | 3' | -55.4 | AC_000018.1 | + | 11074 | 0.74 | 0.191806 |
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Target: 5'- -aGUCGGCugggcGGGUGCCGCUcGGGCGAu -3' miRNA: 3'- uaCAGUCGc----UCUACGGCGAcUCUGCU- -5' |
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| 666 | 3' | -55.4 | AC_000018.1 | + | 9067 | 1.07 | 0.000658 |
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Target: 5'- gAUGUCAGCGAGAUGCCGCUGAGACGAu -3' miRNA: 3'- -UACAGUCGCUCUACGGCGACUCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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