Results 21 - 40 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6660 | 5' | -58.7 | NC_001847.1 | + | 31701 | 0.67 | 0.689708 |
Target: 5'- cUCGGGgGCGgg-GGGAGGcGCggGCCg -3' miRNA: 3'- aAGUCCaCGCggaCCCUCC-CGaaUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 87913 | 0.67 | 0.699822 |
Target: 5'- -aCAGGggcgGgGCgUGGGGcuGGGCgcgGCCg -3' miRNA: 3'- aaGUCCa---CgCGgACCCU--CCCGaa-UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 76393 | 0.67 | 0.679546 |
Target: 5'- cUCGGGggggGCGCCgGGGgccgcggcgGGGGC--ACCc -3' miRNA: 3'- aAGUCCa---CGCGGaCCC---------UCCCGaaUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 102699 | 0.67 | 0.699822 |
Target: 5'- -gCAGG-GCGCC-GGGcGGGCggcgaaagGCCc -3' miRNA: 3'- aaGUCCaCGCGGaCCCuCCCGaa------UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 134514 | 0.67 | 0.689708 |
Target: 5'- cUCGGGgGCGgg-GGGAGGcGCggGCCg -3' miRNA: 3'- aAGUCCaCGCggaCCCUCC-CGaaUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 68064 | 0.68 | 0.587335 |
Target: 5'- cUCGcGGcGCGCCUGcGGcGGGGCgaacGCCg -3' miRNA: 3'- aAGU-CCaCGCGGAC-CC-UCCCGaa--UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 22101 | 0.68 | 0.617022 |
Target: 5'- -gCAGGcGCGCCgGGGGcgacagcGGGCgcGCCc -3' miRNA: 3'- aaGUCCaCGCGGaCCCU-------CCCGaaUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 23011 | 0.68 | 0.60779 |
Target: 5'- cUgGGGUGgGCUgGGGuGGGCUggGCUg -3' miRNA: 3'- aAgUCCACgCGGaCCCuCCCGAa-UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 17793 | 0.68 | 0.628318 |
Target: 5'- gUUCAGGacGgGCCccagcgggcGGGAGGGCgccaUGCCa -3' miRNA: 3'- -AAGUCCa-CgCGGa--------CCCUCCCGa---AUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 125824 | 0.68 | 0.60779 |
Target: 5'- cUgGGGUGgGCUgGGGuGGGCUggGCUg -3' miRNA: 3'- aAgUCCACgCGGaCCCuCCCGAa-UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 124914 | 0.68 | 0.617022 |
Target: 5'- -gCAGGcGCGCCgGGGGcgacagcGGGCgcGCCc -3' miRNA: 3'- aaGUCCaCGCGGaCCCU-------CCCGaaUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 125764 | 0.68 | 0.60779 |
Target: 5'- cUgGGGUGgGCUgGGGuGGGCUggGCUg -3' miRNA: 3'- aAgUCCACgCGGaCCCuCCCGAa-UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 62782 | 0.68 | 0.60779 |
Target: 5'- -cCGGGUcuGgGCCcGGGGGGGCgagAUCg -3' miRNA: 3'- aaGUCCA--CgCGGaCCCUCCCGaa-UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 45090 | 0.68 | 0.60779 |
Target: 5'- cUCGuGGUGCGCCgggacgggGGuGGGGGCagACa -3' miRNA: 3'- aAGU-CCACGCGGa-------CC-CUCCCGaaUGg -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 42609 | 0.68 | 0.597549 |
Target: 5'- -aCAGGgacgGCGCCgccgcgGaGGGGGGCggcagcGCCg -3' miRNA: 3'- aaGUCCa---CGCGGa-----C-CCUCCCGaa----UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 51586 | 0.68 | 0.618049 |
Target: 5'- -cCGGG-GCaGCCgucGGGAGGGCagUGCUc -3' miRNA: 3'- aaGUCCaCG-CGGa--CCCUCCCGa-AUGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 56811 | 0.69 | 0.577153 |
Target: 5'- -aCGGGgccggcGCGCCUGGGggcgcggggagGGGGCggcccaGCCg -3' miRNA: 3'- aaGUCCa-----CGCGGACCC-----------UCCCGaa----UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 108080 | 0.69 | 0.556913 |
Target: 5'- ---cGGUGcCGCCcGGG-GGGCUgcggGCCu -3' miRNA: 3'- aaguCCAC-GCGGaCCCuCCCGAa---UGG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 131740 | 0.69 | 0.536883 |
Target: 5'- -cCGGGcUGCucucccgcuGCUUGcGGAGGGCUUugCg -3' miRNA: 3'- aaGUCC-ACG---------CGGAC-CCUCCCGAAugG- -5' |
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6660 | 5' | -58.7 | NC_001847.1 | + | 14453 | 0.69 | 0.546869 |
Target: 5'- --gGGGUGCGCggccgcggccuCUGGGucuccGGGCUcACCg -3' miRNA: 3'- aagUCCACGCG-----------GACCCu----CCCGAaUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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