Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 6684 | 5' | -55 | NC_001847.1 | + | 100835 | 0.66 | 0.938625 |
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Target: 5'- cCCGGgcGGCUCCGgccagggcCGgagcgCCggCCCg -3' miRNA: 3'- -GGCCuuCCGAGGUa-------GCaca--GGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 128075 | 0.66 | 0.925447 |
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Target: 5'- cCCGGgcGGCUCgCcgCGUG-CCuugguggcgaaagCCCa -3' miRNA: 3'- -GGCCuuCCGAG-GuaGCACaGGua-----------GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 33920 | 0.66 | 0.938625 |
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Target: 5'- uCgGGGAGGCUggCCucccCGUGUgCAggCCCg -3' miRNA: 3'- -GgCCUUCCGA--GGua--GCACAgGUa-GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 83726 | 0.66 | 0.933747 |
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Target: 5'- gCGGgcGGCUCCGUC-UGcgCCG-CCg -3' miRNA: 3'- gGCCuuCCGAGGUAGcACa-GGUaGGg -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 10675 | 0.66 | 0.933747 |
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Target: 5'- gCCGGggGGCUCgCccuccgGUCGcGcCCGggcgcggCCCg -3' miRNA: 3'- -GGCCuuCCGAG-G------UAGCaCaGGUa------GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 127007 | 0.65 | 0.947673 |
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Target: 5'- cCCGcGAGGCU-CGUCGg--CCcgCCCg -3' miRNA: 3'- -GGCcUUCCGAgGUAGCacaGGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 24194 | 0.65 | 0.947673 |
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Target: 5'- cCCGcGAGGCU-CGUCGg--CCcgCCCg -3' miRNA: 3'- -GGCcUUCCGAgGUAGCacaGGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 27611 | 0.65 | 0.947673 |
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Target: 5'- cCCGuuAGGCgcggCCAUCGUuccucGUCCG-CUCg -3' miRNA: 3'- -GGCcuUCCGa---GGUAGCA-----CAGGUaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 9732 | 0.65 | 0.947243 |
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Target: 5'- uCCGGcggcgccGGGGCagucgCCGUCG-GUCCAcCUCg -3' miRNA: 3'- -GGCC-------UUCCGa----GGUAGCaCAGGUaGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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