Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6684 | 5' | -55 | NC_001847.1 | + | 100835 | 0.66 | 0.938625 |
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Target: 5'- cCCGGgcGGCUCCGgccagggcCGgagcgCCggCCCg -3' miRNA: 3'- -GGCCuuCCGAGGUa-------GCaca--GGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 80851 | 0.66 | 0.938625 |
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Target: 5'- uCCGGgcGGCggcgCgCGUCGaggCCGUCCg -3' miRNA: 3'- -GGCCuuCCGa---G-GUAGCacaGGUAGGg -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 113488 | 0.66 | 0.933747 |
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Target: 5'- gCCGGggGGCUCgCccuccgGUCGcGcCCGggcgcggCCCg -3' miRNA: 3'- -GGCCuuCCGAG-G------UAGCaCaGGUa------GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 101883 | 0.66 | 0.928631 |
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Target: 5'- ---aGGGGCUCCAUCGcGUcggCCAgCCCg -3' miRNA: 3'- ggccUUCCGAGGUAGCaCA---GGUaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 102895 | 0.67 | 0.917684 |
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Target: 5'- cCCGGGcccgcucuGGGCUCCGccccuggGUCCGgcgCCCc -3' miRNA: 3'- -GGCCU--------UCCGAGGUagca---CAGGUa--GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 2620 | 0.67 | 0.911853 |
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Target: 5'- gCCGGAGGuGCUUCggCGggaccgCCGUCgCCg -3' miRNA: 3'- -GGCCUUC-CGAGGuaGCaca---GGUAG-GG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 60995 | 0.67 | 0.911257 |
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Target: 5'- aCGGcgccgucGAGGCcgCCAUCGgcgccgcgcGUCaCAUCCCc -3' miRNA: 3'- gGCC-------UUCCGa-GGUAGCa--------CAG-GUAGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 120260 | 0.67 | 0.905787 |
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Target: 5'- gCGGAcGGCcCCGuagcguUCGcUGUCCA-CCCg -3' miRNA: 3'- gGCCUuCCGaGGU------AGC-ACAGGUaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 123502 | 1.13 | 0.002147 |
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Target: 5'- gCCGGAAGGCUCCAUCGUGUCCAUCCCc -3' miRNA: 3'- -GGCCUUCCGAGGUAGCACAGGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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