Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6684 | 5' | -55 | NC_001847.1 | + | 131348 | 0.69 | 0.807184 |
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Target: 5'- cCCGuGccGGCacgCCGUCGUGcagCaCAUCCCg -3' miRNA: 3'- -GGC-CuuCCGa--GGUAGCACa--G-GUAGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 46361 | 0.7 | 0.789241 |
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Target: 5'- aUCGGggGGCUCC-UCGgcgGacaCCAgcaaCCCg -3' miRNA: 3'- -GGCCuuCCGAGGuAGCa--Ca--GGUa---GGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 96487 | 0.7 | 0.751732 |
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Target: 5'- gCGcGAGGGCUCCGuguUCGuUGUCCcgUUCg -3' miRNA: 3'- gGC-CUUCCGAGGU---AGC-ACAGGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 15478 | 0.71 | 0.732311 |
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Target: 5'- cCUGGggGGCcgCCGUUGcccUCCcgCCCg -3' miRNA: 3'- -GGCCuuCCGa-GGUAGCac-AGGuaGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 26691 | 0.72 | 0.662088 |
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Target: 5'- gCCGGcGGGCUCCAgccgcagcUCGgcGUCCAcgUCCa -3' miRNA: 3'- -GGCCuUCCGAGGU--------AGCa-CAGGU--AGGg -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 90336 | 0.72 | 0.658012 |
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Target: 5'- gCCGG-GGGCgcagccgccaaagCCGcCGUcGUCCGUCCCg -3' miRNA: 3'- -GGCCuUCCGa------------GGUaGCA-CAGGUAGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 130255 | 0.72 | 0.648828 |
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Target: 5'- gCGGAugcgacgacaaaauGGGCUUCcgCGUGgcugugcCCGUCCCg -3' miRNA: 3'- gGCCU--------------UCCGAGGuaGCACa------GGUAGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 80653 | 0.83 | 0.19468 |
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Target: 5'- cCCGGccccGGCgccgCCGUCGuUGUCCGUCCCg -3' miRNA: 3'- -GGCCuu--CCGa---GGUAGC-ACAGGUAGGG- -5' |
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| 6684 | 5' | -55 | NC_001847.1 | + | 123502 | 1.13 | 0.002147 |
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Target: 5'- gCCGGAAGGCUCCAUCGUGUCCAUCCCc -3' miRNA: 3'- -GGCCUUCCGAGGUAGCACAGGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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