Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6698 | 5' | -63.5 | NC_001866.1 | + | 2331 | 0.68 | 0.011886 |
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Target: 5'- -gGUugGcGCCGGGCGG-GGCgGcCCGCg -3' miRNA: 3'- caCGugC-CGGUCCGUCgUCGgC-GGCG- -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 2223 | 0.69 | 0.008326 |
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Target: 5'- cUGCACGaCCuGGgAGCcgcGGCCGCCGa -3' miRNA: 3'- cACGUGCcGGuCCgUCG---UCGGCGGCg -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 1311 | 0.7 | 0.007753 |
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Target: 5'- -cGCugGGUgucggGGGUcgcgAGUGGCCGCCGCu -3' miRNA: 3'- caCGugCCGg----UCCG----UCGUCGGCGGCG- -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 1707 | 0.7 | 0.00722 |
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Target: 5'- -cGCugaGCGGCaucGCGGCGGCUGCUGCc -3' miRNA: 3'- caCG---UGCCGgucCGUCGUCGGCGGCG- -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 2232 | 0.71 | 0.005054 |
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Target: 5'- cGUGCAgguagaggcCGGCCgAGGCGGCggGGCCGgCGg -3' miRNA: 3'- -CACGU---------GCCGG-UCCGUCG--UCGGCgGCg -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 1919 | 0.75 | 0.002387 |
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Target: 5'- -gGCgGCGGCCAGGCuggAGCGGCgGCUGg -3' miRNA: 3'- caCG-UGCCGGUCCG---UCGUCGgCGGCg -5' |
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| 6698 | 5' | -63.5 | NC_001866.1 | + | 1665 | 1.11 | 0.000001 |
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Target: 5'- aGUGCACGGCCAGGCAGCAGCCGCCGCg -3' miRNA: 3'- -CACGUGCCGGUCCGUCGUCGGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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