Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6728 | 3' | -46.6 | NC_001875.2 | + | 36447 | 0.66 | 0.999829 |
Target: 5'- cUUGAgGUGGcUGCGGCGcauuggcgcuccGUUGUGCa- -3' miRNA: 3'- cAAUUaUAUC-ACGCCGU------------CAACACGcu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 126994 | 0.66 | 0.999636 |
Target: 5'- --------uGUGCGGCAcUUGUGCa- -3' miRNA: 3'- caauuauauCACGCCGUcAACACGcu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 11875 | 0.66 | 0.999539 |
Target: 5'- --cAGUuu-GUGCGGCagcgcguguuagAGUUGUGCGc -3' miRNA: 3'- caaUUAuauCACGCCG------------UCAACACGCu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 28996 | 0.66 | 0.999539 |
Target: 5'- -gUGGUGgcGUGCGGCAaGUgcgcUGCGGg -3' miRNA: 3'- caAUUAUauCACGCCGU-CAac--ACGCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 109117 | 0.68 | 0.998341 |
Target: 5'- --gGGUAUAGgcggcgacgGCGGCGGUcuUG-GCGAc -3' miRNA: 3'- caaUUAUAUCa--------CGCCGUCA--ACaCGCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 97140 | 0.68 | 0.99758 |
Target: 5'- uUUGAUAacUGGUGCcagGGCuGUUGUGUGc -3' miRNA: 3'- cAAUUAU--AUCACG---CCGuCAACACGCu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 554 | 0.69 | 0.996549 |
Target: 5'- --gGAUGUAGccgGUGGC-GUUGUGCa- -3' miRNA: 3'- caaUUAUAUCa--CGCCGuCAACACGcu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 58270 | 0.69 | 0.99518 |
Target: 5'- gGUUGGUcgggagGUGGcUGCGGCGGUUGUuGgGGc -3' miRNA: 3'- -CAAUUA------UAUC-ACGCCGUCAACA-CgCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 13994 | 0.69 | 0.994346 |
Target: 5'- uUUGAccUGGUgGUGGCGGUUGaGCGAa -3' miRNA: 3'- cAAUUauAUCA-CGCCGUCAACaCGCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 831 | 0.7 | 0.988277 |
Target: 5'- -----gGUGGUGgGGCGGUgGUGCa- -3' miRNA: 3'- caauuaUAUCACgCCGUCAaCACGcu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 66296 | 0.72 | 0.969411 |
Target: 5'- --aGGUGUGGUGCGuGUAcaUUGUGCGAc -3' miRNA: 3'- caaUUAUAUCACGC-CGUc-AACACGCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 40214 | 0.72 | 0.966047 |
Target: 5'- -----cGUAGUGCGGCGuGUUGU-CGAg -3' miRNA: 3'- caauuaUAUCACGCCGU-CAACAcGCU- -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 17427 | 0.75 | 0.882924 |
Target: 5'- -------cAGUGCGGCGGaUUGUGCGc -3' miRNA: 3'- caauuauaUCACGCCGUC-AACACGCu -5' |
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6728 | 3' | -46.6 | NC_001875.2 | + | 749 | 1.1 | 0.016493 |
Target: 5'- gGUUAAUAUAGUGCGGCAGUUGUGCGAc -3' miRNA: 3'- -CAAUUAUAUCACGCCGUCAACACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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