Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6729 | 3' | -62.8 | NC_001875.2 | + | 68704 | 0.66 | 0.576362 |
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Target: 5'- gGAC-CcGUGCGCCggccgCGcGCCCgGCCg -3' miRNA: 3'- gUUGcGaCACGCGGa----GCcCGGGgCGG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 42190 | 0.66 | 0.582223 |
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Target: 5'- gCAGCGCgGgcggcagaucgugGUGCCgguuaaCGGG-CCCGCCg -3' miRNA: 3'- -GUUGCGaCa------------CGCGGa-----GCCCgGGGCGG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 10865 | 0.66 | 0.586137 |
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Target: 5'- aCGACGCgGUGgcCGCgUCGguGGCgCUGCCg -3' miRNA: 3'- -GUUGCGaCAC--GCGgAGC--CCGgGGCGG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 36442 | 0.66 | 0.586137 |
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Target: 5'- gGugGCUGcgGCGCaUUGGcGCUCCGUUg -3' miRNA: 3'- gUugCGACa-CGCGgAGCC-CGGGGCGG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 131502 | 0.66 | 0.586137 |
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Target: 5'- -cACGUUGUGCGCCUguuuuUGGcGCUCCa-- -3' miRNA: 3'- guUGCGACACGCGGA-----GCC-CGGGGcgg -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 130615 | 0.66 | 0.595942 |
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Target: 5'- aCGGCGCgugcuagcgGUGCGUCagCGGcGCCCUGgUg -3' miRNA: 3'- -GUUGCGa--------CACGCGGa-GCC-CGGGGCgG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 62556 | 0.66 | 0.595942 |
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Target: 5'- aAACGUUGUGCGgg-UGGacuuGCCgCCGCCa -3' miRNA: 3'- gUUGCGACACGCggaGCC----CGG-GGCGG- -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 109878 | 0.66 | 0.595942 |
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Target: 5'- uGGCGCUG-GCGCCgcccauCCCCGCg -3' miRNA: 3'- gUUGCGACaCGCGGagccc-GGGGCGg -5' |
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| 6729 | 3' | -62.8 | NC_001875.2 | + | 40239 | 0.66 | 0.595942 |
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Target: 5'- uGGCGCgucaGCGCCgc-GGCCUCGUCg -3' miRNA: 3'- gUUGCGaca-CGCGGagcCCGGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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