Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
673 | 5' | -50.6 | AC_000018.1 | + | 26872 | 0.66 | 0.861917 |
Target: 5'- cGGCGggaaUCACGuCACCCuugGCACcuguccuUUGCc -3' miRNA: 3'- -CCGU----AGUGC-GUGGGucaUGUGu------AACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 21771 | 0.66 | 0.834544 |
Target: 5'- gGGC-UCcUGCGCCgCAGUGC-CcUUGCu -3' miRNA: 3'- -CCGuAGuGCGUGG-GUCAUGuGuAACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 10034 | 0.66 | 0.824921 |
Target: 5'- --uGUCugGUGCCCAGgGCGCAcgaGCu -3' miRNA: 3'- ccgUAGugCGUGGGUCaUGUGUaa-CG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 5199 | 0.66 | 0.824921 |
Target: 5'- gGGU---GCGCGCCUGGUuggGCGC-UUGCg -3' miRNA: 3'- -CCGuagUGCGUGGGUCA---UGUGuAACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 10291 | 0.66 | 0.824921 |
Target: 5'- cGCGUaCGCGguUCCAGauguUGCGCAgcgGCa -3' miRNA: 3'- cCGUA-GUGCguGGGUC----AUGUGUaa-CG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 10108 | 0.67 | 0.773588 |
Target: 5'- uGCAggUGCGCACCaGGUACugGUaGCc -3' miRNA: 3'- cCGUa-GUGCGUGGgUCAUGugUAaCG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 10120 | 0.67 | 0.762769 |
Target: 5'- cGCAUacuCGCGCCUcaAGUACACcgagcucgUGCg -3' miRNA: 3'- cCGUAgu-GCGUGGG--UCAUGUGua------ACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 24483 | 0.68 | 0.751797 |
Target: 5'- gGGCAUCgGCGCGggUgCGGUugGCGcgGCa -3' miRNA: 3'- -CCGUAG-UGCGU--GgGUCAugUGUaaCG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 10171 | 0.68 | 0.706663 |
Target: 5'- cGGCuaccaGUACCUGGUGCGCAccUGCa -3' miRNA: 3'- -CCGuagugCGUGGGUCAUGUGUa-ACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 12696 | 0.69 | 0.683541 |
Target: 5'- cGGCcUCGCGCACaucggucaccauCCuGUccagguugguuuGCACGUUGCu -3' miRNA: 3'- -CCGuAGUGCGUG------------GGuCA------------UGUGUAACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 16317 | 0.69 | 0.660196 |
Target: 5'- cGGCcagCGCGUGCCC-GUGCGCAcucGCc -3' miRNA: 3'- -CCGua-GUGCGUGGGuCAUGUGUaa-CG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 12082 | 0.69 | 0.648472 |
Target: 5'- uGGCGcUCAC-CGCgCGGUGCAuccuguCAUUGCg -3' miRNA: 3'- -CCGU-AGUGcGUGgGUCAUGU------GUAACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 5444 | 0.7 | 0.624986 |
Target: 5'- uGCAUC-CGCACCgCAGgagGCGCAgacgGUu -3' miRNA: 3'- cCGUAGuGCGUGG-GUCa--UGUGUaa--CG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 15850 | 0.7 | 0.609729 |
Target: 5'- aGGCGccCGCaaacgcucuacccaGCACCCuGUGCGCGUUcGCg -3' miRNA: 3'- -CCGUa-GUG--------------CGUGGGuCAUGUGUAA-CG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 22975 | 0.78 | 0.224391 |
Target: 5'- uGCAUCcaugagcccacagaGCGCACCCGGUGCACucccaacuAUUGUg -3' miRNA: 3'- cCGUAG--------------UGCGUGGGUCAUGUG--------UAACG- -5' |
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673 | 5' | -50.6 | AC_000018.1 | + | 16347 | 1.14 | 0.000793 |
Target: 5'- uGGCAUCACGCACCCAGUACACAUUGCc -3' miRNA: 3'- -CCGUAGUGCGUGGGUCAUGUGUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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