miRNA: 3'- -CGCGCAU-----UUGG-ACC-UG-CAaCUGgCGG- -5'
miRNA: 3'- -CGcgCAu-UUGGa-CCuGCAACUGGCGG- -5'
miRNA: 3'- -CGC-GCAUuug-GACCUGCaa-CUGGCGg -5'
Results 121 - 123 of 123 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6731 | 3' | -52.6 | NC_001875.2 | + | 37416 | 0.66 | 0.980705 |
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Target: 5'- gGCGCGUGgaagaGGCCgUGGcGCaGUcGGCgGCCa -3' miRNA: 3'- -CGCGCAU-----UUGG-ACC-UG-CAaCUGgCGG- -5' |
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| 6731 | 3' | -52.6 | NC_001875.2 | + | 58954 | 0.66 | 0.980705 |
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Target: 5'- aGCuuGUucGACUgaGGcCGUUGGCCGCCc -3' miRNA: 3'- -CGcgCAu-UUGGa-CCuGCAACUGGCGG- -5' |
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| 6731 | 3' | -52.6 | NC_001875.2 | + | 79782 | 0.66 | 0.980705 |
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Target: 5'- cGCGcCGUGcaaaCUGGACGcgcGACUGCg -3' miRNA: 3'- -CGC-GCAUuug-GACCUGCaa-CUGGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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