miRNA: 3'- aCUGaCGUCCG-GGCGAACGCa--GUGUu -5'
miRNA: 3'- -ACUGACGUCCGGgCGaac-GCAG---UGUU- -5'
miRNA: 3'- acUGACGUCCGGgCGaACGCaGUGuu -5'
miRNA: 3'- acuGACGu--CCGG-GCGAACGCAGUGUU- -5'
miRNA: 3'- acUGACGUCCGGg-CGA--------ACGCaGUGUu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6734 | 5' | -57.6 | NC_001875.2 | + | 112121 | 0.67 | 0.682929 |
|
Target: 5'- cGGC-GCGGGCgCCGUUUGCGaaaCGCGu -3' miRNA: 3'- aCUGaCGUCCG-GGCGAACGCa--GUGUu -5' |
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| 6734 | 5' | -57.6 | NC_001875.2 | + | 117190 | 0.7 | 0.537761 |
|
Target: 5'- gUGGCUGCAGGCCgGCaccuCGUCgaaGCGAa -3' miRNA: 3'- -ACUGACGUCCGGgCGaac-GCAG---UGUU- -5' |
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| 6734 | 5' | -57.6 | NC_001875.2 | + | 117931 | 0.71 | 0.497809 |
|
Target: 5'- -aGCUGCAGGCCgGCgUGCG-CGCc- -3' miRNA: 3'- acUGACGUCCGGgCGaACGCaGUGuu -5' |
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| 6734 | 5' | -57.6 | NC_001875.2 | + | 125584 | 0.7 | 0.52766 |
|
Target: 5'- --gUUGCcacGGCCuCGUUUGCGUCACAGu -3' miRNA: 3'- acuGACGu--CCGG-GCGAACGCAGUGUU- -5' |
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| 6734 | 5' | -57.6 | NC_001875.2 | + | 129523 | 0.66 | 0.743882 |
|
Target: 5'- -aGCUGCAGGCCgaGCUggccgagcUGCG-CGCGg -3' miRNA: 3'- acUGACGUCCGGg-CGA--------ACGCaGUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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