Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6738 | 3' | -57.4 | NC_001875.2 | + | 85249 | 0.66 | 0.838201 |
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Target: 5'- cGCGGGuuccgcgcucggcGCGGACAC-GAGCG-C-CCCGa -3' miRNA: 3'- -UGCCC-------------CGUCUGUGuCUUGCaGaGGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 62606 | 0.66 | 0.822378 |
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Target: 5'- --cGGGCAagucGGCACAGcgcGCGUCgCCCGa -3' miRNA: 3'- ugcCCCGU----CUGUGUCu--UGCAGaGGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 30920 | 0.67 | 0.759186 |
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Target: 5'- gUGGGaGCGaGCGCGgcGAGCGUCUCCgCGc -3' miRNA: 3'- uGCCC-CGUcUGUGU--CUUGCAGAGG-GC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 63203 | 0.68 | 0.749642 |
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Target: 5'- uUGcGGGCAG-CGCAGcuuuACGUCgCCCGc -3' miRNA: 3'- uGC-CCCGUCuGUGUCu---UGCAGaGGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 19342 | 0.68 | 0.730258 |
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Target: 5'- uACGuGGGCGugguGCGCGGAGCGUUggCCGa -3' miRNA: 3'- -UGC-CCCGUc---UGUGUCUUGCAGagGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 29370 | 0.68 | 0.730258 |
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Target: 5'- gACGGGGUGGACAUcgauuGGGA-GUUUCCgGg -3' miRNA: 3'- -UGCCCCGUCUGUG-----UCUUgCAGAGGgC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 85306 | 0.68 | 0.720436 |
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Target: 5'- cGCGGGcGCGGGCAUugccgcGGGCG-CUCUCGc -3' miRNA: 3'- -UGCCC-CGUCUGUGu-----CUUGCaGAGGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 108749 | 0.69 | 0.660239 |
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Target: 5'- gGCGGGGCAGcugcACGCGGcGCGggUUgCCGg -3' miRNA: 3'- -UGCCCCGUC----UGUGUCuUGCa-GAgGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 83566 | 0.72 | 0.500065 |
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Target: 5'- cACGcGGCGGACACAuGAgGUgUCCCGg -3' miRNA: 3'- -UGCcCCGUCUGUGUcUUgCAgAGGGC- -5' |
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| 6738 | 3' | -57.4 | NC_001875.2 | + | 117273 | 1.09 | 0.002015 |
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Target: 5'- gACGGGGCAGACACAGAACGUCUCCCGa -3' miRNA: 3'- -UGCCCCGUCUGUGUCUUGCAGAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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