Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
674 | 3' | -58 | AC_000018.1 | + | 16047 | 0.66 | 0.429745 |
Target: 5'- -----aGaCGCCCGCGCCuaUGCuCGCc -3' miRNA: 3'- gugaaaC-GCGGGCGCGGugACGuGCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 21613 | 0.67 | 0.382572 |
Target: 5'- aGCU---CGCCUGCGCCauagucaacACUGCcggACGCg -3' miRNA: 3'- gUGAaacGCGGGCGCGG---------UGACG---UGCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 16844 | 0.67 | 0.347398 |
Target: 5'- aGCaagUGCuGCCCGUGCC--UGCGCGg -3' miRNA: 3'- gUGaa-ACG-CGGGCGCGGugACGUGCg -5' |
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674 | 3' | -58 | AC_000018.1 | + | 10994 | 0.68 | 0.330698 |
Target: 5'- gGCgg-GCuGuCCCGCGCCGCUG-ACGg -3' miRNA: 3'- gUGaaaCG-C-GGGCGCGGUGACgUGCg -5' |
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674 | 3' | -58 | AC_000018.1 | + | 11266 | 0.68 | 0.330698 |
Target: 5'- gGCgg-GCuGuCCCGCGCCGCUG-ACGg -3' miRNA: 3'- gUGaaaCG-C-GGGCGCGGUGACgUGCg -5' |
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674 | 3' | -58 | AC_000018.1 | + | 9824 | 0.68 | 0.306771 |
Target: 5'- gCACUUcuucuuCGCCCGCucuGCCA-UGCAUGCg -3' miRNA: 3'- -GUGAAac----GCGGGCG---CGGUgACGUGCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 12979 | 0.68 | 0.299096 |
Target: 5'- aACUcc-CGCCUGCuGCUGCUGCugGUa -3' miRNA: 3'- gUGAaacGCGGGCG-CGGUGACGugCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 12104 | 0.71 | 0.195957 |
Target: 5'- uCGCUca--GCUCGCGCCucCUGCugGCg -3' miRNA: 3'- -GUGAaacgCGGGCGCGGu-GACGugCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 4538 | 0.72 | 0.170756 |
Target: 5'- aACgugUU-CGCCCGUGCCGCUGCccACGg -3' miRNA: 3'- gUGa--AAcGCGGGCGCGGUGACG--UGCg -5' |
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674 | 3' | -58 | AC_000018.1 | + | 16269 | 0.76 | 0.085837 |
Target: 5'- -----cGCGCCUGCgggaccuGCCGCUGCugGCa -3' miRNA: 3'- gugaaaCGCGGGCG-------CGGUGACGugCG- -5' |
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674 | 3' | -58 | AC_000018.1 | + | 16640 | 1.11 | 0.000178 |
Target: 5'- gCACUUUGCGCCCGCGCCACUGCACGCg -3' miRNA: 3'- -GUGAAACGCGGGCGCGGUGACGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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