Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
674 | 5' | -53.2 | AC_000018.1 | + | 5059 | 0.66 | 0.744552 |
Target: 5'- cGGCucGAGCUCuuGCaACAGACUCUuuCCa -3' miRNA: 3'- cCCG--CUUGAGcgCG-UGUUUGAGGu-GG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 10714 | 0.66 | 0.744552 |
Target: 5'- uGGCGAuuaauucuccagACUaCGgGCGCGAgccACUCCcaGCCc -3' miRNA: 3'- cCCGCU------------UGA-GCgCGUGUU---UGAGG--UGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 21369 | 0.66 | 0.744552 |
Target: 5'- aGGGUGGGCUCaucCAUggGaUCCACCu -3' miRNA: 3'- -CCCGCUUGAGcgcGUGuuUgAGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 24317 | 0.66 | 0.733673 |
Target: 5'- aGGCGAaaagagguucaGCUCGUagGCGCGgcuaAGCUCUuCCa -3' miRNA: 3'- cCCGCU-----------UGAGCG--CGUGU----UUGAGGuGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 9216 | 0.66 | 0.722684 |
Target: 5'- cGGGgGAuUUCGaGCGCGAgguGCgcaCCACCa -3' miRNA: 3'- -CCCgCUuGAGCgCGUGUU---UGa--GGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 26006 | 0.66 | 0.700427 |
Target: 5'- uGGGgGAAcCUUGUGUugA-GCUCCucACCg -3' miRNA: 3'- -CCCgCUU-GAGCGCGugUuUGAGG--UGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 12729 | 0.67 | 0.650595 |
Target: 5'- uGGCGccgcgcuggaaccGCUCGCGCugAGACaCgGCCu -3' miRNA: 3'- cCCGCu------------UGAGCGCGugUUUGaGgUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 13162 | 0.67 | 0.648314 |
Target: 5'- uGGGUGAuuucuugauaggucuGCUCGUcCACcuGGCUCUGCCc -3' miRNA: 3'- -CCCGCU---------------UGAGCGcGUGu-UUGAGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 19485 | 0.67 | 0.64375 |
Target: 5'- aGGCGAAaaaGCGUcaGCAGcuuCUCUACCa -3' miRNA: 3'- cCCGCUUgagCGCG--UGUUu--GAGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 6186 | 0.68 | 0.6369 |
Target: 5'- cGGCGAuguuaagcuggacguACUCGCGCGCcacacauUUCCAUUc -3' miRNA: 3'- cCCGCU---------------UGAGCGCGUGuuu----GAGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 18207 | 0.68 | 0.632333 |
Target: 5'- uGGUGA---CGCGCGCAGACgagCCcCCu -3' miRNA: 3'- cCCGCUugaGCGCGUGUUUGa--GGuGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 8216 | 0.68 | 0.620917 |
Target: 5'- -uGCGcGCUCuCGUccuCGAGCUCCGCCc -3' miRNA: 3'- ccCGCuUGAGcGCGu--GUUUGAGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 8153 | 0.68 | 0.586772 |
Target: 5'- gGGGCgGAGCUCgaggacgagaGCGCGCAGGCcggagcugUCCAg- -3' miRNA: 3'- -CCCG-CUUGAG----------CGCGUGUUUG--------AGGUgg -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 25538 | 0.7 | 0.498141 |
Target: 5'- aGGCGAACagGUaGCAguGGCUCCAUa -3' miRNA: 3'- cCCGCUUGagCG-CGUguUUGAGGUGg -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 11052 | 0.7 | 0.487454 |
Target: 5'- cGGGCGAuggUGCGCcCAGGCg-CGCCa -3' miRNA: 3'- -CCCGCUugaGCGCGuGUUUGagGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 11324 | 0.7 | 0.487454 |
Target: 5'- cGGGCGAuggUGCGCcCAGGCg-CGCCa -3' miRNA: 3'- -CCCGCUugaGCGCGuGUUUGagGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 16382 | 0.7 | 0.487454 |
Target: 5'- gGGGCGAG--UGCGCACGGGCaCgCGCUg -3' miRNA: 3'- -CCCGCUUgaGCGCGUGUUUGaG-GUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 33587 | 0.7 | 0.487454 |
Target: 5'- aGGGUGAugUCugGCGCAUGAugUCgAgCg -3' miRNA: 3'- -CCCGCUugAG--CGCGUGUUugAGgUgG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 8868 | 0.71 | 0.425812 |
Target: 5'- cGGGaucuCUCGCGCGCAuaa-CCACCu -3' miRNA: 3'- -CCCgcuuGAGCGCGUGUuugaGGUGG- -5' |
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674 | 5' | -53.2 | AC_000018.1 | + | 16105 | 0.71 | 0.425812 |
Target: 5'- cGGCGAGCauaggCGCGgGCGucugCCACCa -3' miRNA: 3'- cCCGCUUGa----GCGCgUGUuugaGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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