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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6740 | 5' | -55.7 | NC_001875.2 | + | 28833 | 0.66 | 0.88658 |
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Target: 5'- cGUCaggguGGCCGaca-CGGACGGCGGGc -3' miRNA: 3'- -CAGgu---CCGGCaaagGUCUGUUGCCCa -5' |
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| 6740 | 5' | -55.7 | NC_001875.2 | + | 100666 | 0.66 | 0.879496 |
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Target: 5'- cGUUCuGGCCGUagCUguGGugAGCGGGc -3' miRNA: 3'- -CAGGuCCGGCAaaGG--UCugUUGCCCa -5' |
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| 6740 | 5' | -55.7 | NC_001875.2 | + | 78653 | 0.67 | 0.833276 |
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Target: 5'- cGUCgAGGCCGUgcaagcgUCCAuGACcgagcgauaaauugcGCGGGUg -3' miRNA: 3'- -CAGgUCCGGCAa------AGGU-CUGu--------------UGCCCA- -5' |
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| 6740 | 5' | -55.7 | NC_001875.2 | + | 121483 | 0.67 | 0.832432 |
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Target: 5'- -gCCAGGCCGUagCCcGGCGcaauuugaaucACGGGc -3' miRNA: 3'- caGGUCCGGCAaaGGuCUGU-----------UGCCCa -5' |
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| 6740 | 5' | -55.7 | NC_001875.2 | + | 30259 | 0.69 | 0.759552 |
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Target: 5'- -gCCAGGUCGacagucCCGGcCAACGGGUu -3' miRNA: 3'- caGGUCCGGCaaa---GGUCuGUUGCCCA- -5' |
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| 6740 | 5' | -55.7 | NC_001875.2 | + | 115362 | 1.08 | 0.00294 |
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Target: 5'- cGUCCAGGCCGUUUCCAGACAACGGGUa -3' miRNA: 3'- -CAGGUCCGGCAAAGGUCUGUUGCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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