miRNA: 3'- caUGACGUUgCGGCGgcaCAGGUGUU- -5'
miRNA: 3'- caUGACGuug----CGGCGGCacaGGUGUU- -5'
miRNA: 3'- caUGa---CGUUGCGG----CGGCACAGgUGUu -5'
miRNA: 3'- cAUGACGUUGCG-----------GCGGCAc-AGGUGUu -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6742 | 5' | -55.9 | NC_001875.2 | + | 928 | 0.66 | 0.874525 |
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Target: 5'- -gACUGCAGgGCCGC---GUCCAUGAu -3' miRNA: 3'- caUGACGUUgCGGCGgcaCAGGUGUU- -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 58049 | 0.66 | 0.874525 |
|
Target: 5'- -cGCUGCcguuccaGCCGCCGccgCCACAAc -3' miRNA: 3'- caUGACGuug----CGGCGGCacaGGUGUU- -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 61604 | 0.66 | 0.874525 |
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Target: 5'- -gGCgccgGCGGCGCUucggGCUGUGUCgGCGg -3' miRNA: 3'- caUGa---CGUUGCGG----CGGCACAGgUGUu -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 61480 | 0.66 | 0.874525 |
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Target: 5'- uUGCUGCAGCGCgucgauuaucaCGUCGUuuUCCGCGu -3' miRNA: 3'- cAUGACGUUGCG-----------GCGGCAc-AGGUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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