miRNA: 3'- caUGACGUUgCGGCGGC-----ACa----GGUGUU- -5'
miRNA: 3'- -CAUgACGuUGCG-GCGGCACaGGUGUu -5'
miRNA: 3'- cAUGACGU---------------UGCGGCGGCacaGGUGuu -5'
miRNA: 3'- caUGACGUUgCGGCGgcaCAGGUGUU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6742 | 5' | -55.9 | NC_001875.2 | + | 5046 | 0.67 | 0.799161 |
|
Target: 5'- -gGCUGCAAaGCCGCCGcagcuUGcacagCCGCAGu -3' miRNA: 3'- caUGACGUUgCGGCGGC-----ACa----GGUGUU- -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 2289 | 0.66 | 0.834616 |
|
Target: 5'- cGUGaUGC-GCGCgCGCCGUGgCCGCGc -3' miRNA: 3'- -CAUgACGuUGCG-GCGGCACaGGUGUu -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 1717 | 0.68 | 0.765061 |
|
Target: 5'- aUGCUGCGggcgcgacaccggucGCGCCGCCGccgCCGCc- -3' miRNA: 3'- cAUGACGU---------------UGCGGCGGCacaGGUGuu -5' |
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| 6742 | 5' | -55.9 | NC_001875.2 | + | 928 | 0.66 | 0.874525 |
|
Target: 5'- -gACUGCAGgGCCGC---GUCCAUGAu -3' miRNA: 3'- caUGACGUUgCGGCGgcaCAGGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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