Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6744 | 3' | -52.8 | NC_001875.2 | + | 77264 | 0.66 | 0.955227 |
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Target: 5'- -cGCGCCgccggGCgaaGCG--GCCGCCg -3' miRNA: 3'- gaCGCGGaaa--CGaa-CGUuaUGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 99436 | 0.66 | 0.955227 |
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Target: 5'- uUGCGCCgucGCccacaacuuUUGCGucGCCGCCc -3' miRNA: 3'- gACGCGGaaaCG---------AACGUuaUGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 17307 | 0.66 | 0.95801 |
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Target: 5'- -aGCGCCgccgauguuaaagaGCUgcugGCGGUugCGCCg -3' miRNA: 3'- gaCGCGGaaa-----------CGAa---CGUUAugGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 77675 | 0.66 | 0.959165 |
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Target: 5'- -aGCGCCgcUGUccgagcgcguauUUGCGcgACUGCCg -3' miRNA: 3'- gaCGCGGaaACG------------AACGUuaUGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 45111 | 0.66 | 0.959165 |
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Target: 5'- -cGCGCCUUcaugUGCgacgaGCAGUGuCgGCCg -3' miRNA: 3'- gaCGCGGAA----ACGaa---CGUUAU-GgCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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