Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6746 | 5' | -48.2 | NC_001875.2 | + | 36147 | 0.68 | 0.994671 |
Target: 5'- aCGCGCGUUugcAGcAAGUgGGCcagcuuaAGCAUGu -3' miRNA: 3'- -GCGCGCAAu--UCaUUCAgCCG-------UUGUAC- -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 33571 | 0.71 | 0.956825 |
Target: 5'- cCGCGgGgcaccaugcccGAGUCGGCGACGUGg -3' miRNA: 3'- -GCGCgCaauuca-----UUCAGCCGUUGUAC- -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 31984 | 0.69 | 0.985957 |
Target: 5'- gCGCGCGUacggccggUuuGUGAG-CGGCGGCGa- -3' miRNA: 3'- -GCGCGCA--------AuuCAUUCaGCCGUUGUac -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 31901 | 0.66 | 0.998438 |
Target: 5'- aGCGCGUUGgcaaAGUAcauGG-CGuGCAGCAUc -3' miRNA: 3'- gCGCGCAAU----UCAU---UCaGC-CGUUGUAc -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 31866 | 0.66 | 0.999108 |
Target: 5'- uCGCGCGUggacuggcgccGGUugcAAGUUGGCGACc-- -3' miRNA: 3'- -GCGCGCAau---------UCA---UUCAGCCGUUGuac -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 24052 | 0.68 | 0.994753 |
Target: 5'- aCGCGCGUUAccggcgucuGGUucGUuuugggCGGCGGCGUu -3' miRNA: 3'- -GCGCGCAAU---------UCAuuCA------GCCGUUGUAc -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 21043 | 0.73 | 0.923923 |
Target: 5'- uGCGCGgcguuGUAuccguGGUCGGCGGCGUu -3' miRNA: 3'- gCGCGCaauu-CAU-----UCAGCCGUUGUAc -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 11926 | 0.66 | 0.998714 |
Target: 5'- aGCGUGUUcugcacccagGGGUugAGGUCGcGCAGCGa- -3' miRNA: 3'- gCGCGCAA----------UUCA--UUCAGC-CGUUGUac -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 10144 | 0.67 | 0.996782 |
Target: 5'- gGCGUGUUccGcAAGcgCGGCGugGUGg -3' miRNA: 3'- gCGCGCAAuuCaUUCa-GCCGUugUAC- -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 6212 | 0.7 | 0.971846 |
Target: 5'- aGCGCGcaaUUGcGUccGUCGGCGACAc- -3' miRNA: 3'- gCGCGC---AAUuCAuuCAGCCGUUGUac -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 5549 | 0.74 | 0.884209 |
Target: 5'- gCGCGCGUUAAGgugccAGUcaCGGCGACuGUGc -3' miRNA: 3'- -GCGCGCAAUUCau---UCA--GCCGUUG-UAC- -5' |
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6746 | 5' | -48.2 | NC_001875.2 | + | 3163 | 0.7 | 0.977366 |
Target: 5'- uCGgGCGgcgagUAGGUGAGcCGGCGGCc-- -3' miRNA: 3'- -GCgCGCa----AUUCAUUCaGCCGUUGuac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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