Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 675 | 5' | -57.9 | AC_000018.1 | + | 16174 | 0.67 | 0.362414 |
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Target: 5'- --aUGCUUagAGcGGCCAGACgCGCGGCu -3' miRNA: 3'- uagGCGAAggUU-CCGGUCUG-GCGUCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 10468 | 0.67 | 0.371319 |
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Target: 5'- -cCCGgUUCgAaucucggaucAGGCUGGAgCCGCAGCu -3' miRNA: 3'- uaGGCgAAGgU----------UCCGGUCU-GGCGUCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 18413 | 0.67 | 0.371319 |
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Target: 5'- -cCCGUUgCCGuaGCCAGACCGCguccugggGGCa -3' miRNA: 3'- uaGGCGAaGGUucCGGUCUGGCG--------UCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 17673 | 0.67 | 0.380372 |
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Target: 5'- ---aGCUUCCGuGGCgguuCAGGCCucGCAGCg -3' miRNA: 3'- uaggCGAAGGUuCCG----GUCUGG--CGUCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 26231 | 0.66 | 0.408403 |
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Target: 5'- cUCCGC-UCC-GGGUCGGGggguCCaGCGGCg -3' miRNA: 3'- uAGGCGaAGGuUCCGGUCU----GG-CGUCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 27782 | 0.72 | 0.163712 |
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Target: 5'- aAUCCGCacUCCGuGGGCCGGACCcugagGguGCu -3' miRNA: 3'- -UAGGCGa-AGGU-UCCGGUCUGG-----CguCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 14328 | 0.67 | 0.345052 |
|
Target: 5'- cUCC-CUUCCGuaGGcGCCAGguACCGCGGg -3' miRNA: 3'- uAGGcGAAGGU--UC-CGGUC--UGGCGUCg -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 28689 | 0.67 | 0.362414 |
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Target: 5'- uGUCUGCUcaugaaCAuGGCCAGAUCGCAc- -3' miRNA: 3'- -UAGGCGAag----GUuCCGGUCUGGCGUcg -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 11583 | 0.67 | 0.362414 |
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Target: 5'- gGUCUGC-UCguAGGCUGagacagguuGGCCGCGGCc -3' miRNA: 3'- -UAGGCGaAGguUCCGGU---------CUGGCGUCG- -5' |
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| 675 | 5' | -57.9 | AC_000018.1 | + | 23711 | 1.08 | 0.000279 |
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Target: 5'- cAUCCGCUUCCAAGGCCAGACCGCAGCc -3' miRNA: 3'- -UAGGCGAAGGUUCCGGUCUGGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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