Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6759 | 3' | -51.5 | NC_001875.2 | + | 110271 | 0.67 | 0.973967 |
Target: 5'- -cCGgcUGGccgcggagcgcgcGGGCUGCCCCGUCGcCGa -3' miRNA: 3'- gaGCaaACU-------------CCUGAUGGGGUAGCuGU- -5' |
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6759 | 3' | -51.5 | NC_001875.2 | + | 33777 | 0.68 | 0.953922 |
Target: 5'- gUCGUUgaggccGGGGuggucuaggcgcACUugCCCGUCGGCGc -3' miRNA: 3'- gAGCAAa-----CUCC------------UGAugGGGUAGCUGU- -5' |
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6759 | 3' | -51.5 | NC_001875.2 | + | 36914 | 0.68 | 0.945246 |
Target: 5'- -gCGUUUGuuGGACUGCugCCCAaCGGCGa -3' miRNA: 3'- gaGCAAACu-CCUGAUG--GGGUaGCUGU- -5' |
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6759 | 3' | -51.5 | NC_001875.2 | + | 105040 | 0.69 | 0.924855 |
Target: 5'- aCUUGUUUGAcGGACacACCaCCcUCGACGg -3' miRNA: 3'- -GAGCAAACU-CCUGa-UGG-GGuAGCUGU- -5' |
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6759 | 3' | -51.5 | NC_001875.2 | + | 48885 | 0.71 | 0.864191 |
Target: 5'- gUCGcc-GAGGACgcGCCCCcgCGACGa -3' miRNA: 3'- gAGCaaaCUCCUGa-UGGGGuaGCUGU- -5' |
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6759 | 3' | -51.5 | NC_001875.2 | + | 98076 | 1.1 | 0.006038 |
Target: 5'- aCUCGUUUGAGGACUACCCCAUCGACAa -3' miRNA: 3'- -GAGCAAACUCCUGAUGGGGUAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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