Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 677 | 5' | -52.2 | AC_000018.1 | + | 23909 | 0.67 | 0.681593 |
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Target: 5'- gAUGaugaGGAGGaGGAGGUcGACGCAGc -3' miRNA: 3'- aUACcg--UCUCCaUCUCCAuCUGUGUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 31608 | 0.67 | 0.666349 |
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Target: 5'- --gGGUguugGGAGGUgcuuucaucuucauAGGGGUAGACAgGGu -3' miRNA: 3'- auaCCG----UCUCCA--------------UCUCCAUCUGUgUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 30902 | 0.67 | 0.658112 |
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Target: 5'- aAUGGCAgGAGGUAGugaAGGUuGugGCu- -3' miRNA: 3'- aUACCGU-CUCCAUC---UCCAuCugUGuc -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 26053 | 0.67 | 0.646324 |
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Target: 5'- --aGGCAGAGGaggAGAuGGaAGAUugGGa -3' miRNA: 3'- auaCCGUCUCCa--UCU-CCaUCUGugUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 10319 | 0.68 | 0.622715 |
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Target: 5'- --cGGCAuGAaGUAGuucauGGUAGGCACGGu -3' miRNA: 3'- auaCCGU-CUcCAUCu----CCAUCUGUGUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 2291 | 0.68 | 0.599143 |
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Target: 5'- gGUGGCAuGAGGUucAGAGcGaAGGCAgGGa -3' miRNA: 3'- aUACCGU-CUCCA--UCUC-CaUCUGUgUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 26086 | 0.68 | 0.5874 |
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Target: 5'- --aGGCAGAGG---AGGUGGACAg-- -3' miRNA: 3'- auaCCGUCUCCaucUCCAUCUGUguc -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 23940 | 0.74 | 0.28681 |
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Target: 5'- --gGGUAGAGGUGGuGGUagcuGGACugGGu -3' miRNA: 3'- auaCCGUCUCCAUCuCCA----UCUGugUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 23624 | 0.8 | 0.107301 |
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Target: 5'- --gGGCAGAGGUGGAGGc-GACugAGa -3' miRNA: 3'- auaCCGUCUCCAUCUCCauCUGugUC- -5' |
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| 677 | 5' | -52.2 | AC_000018.1 | + | 25191 | 1.07 | 0.00131 |
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Target: 5'- gUAUGGCAGAGGUAGAGGUAGACACAGu -3' miRNA: 3'- -AUACCGUCUCCAUCUCCAUCUGUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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