Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6770 | 5' | -50.9 | NC_001875.2 | + | 19108 | 0.7 | 0.943367 |
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Target: 5'- gCCGUGU---UGCuGUGCGCGCGAc-- -3' miRNA: 3'- -GGUACAacuACGcCAUGCGCGCUucc -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 67141 | 0.71 | 0.891263 |
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Target: 5'- gCCGUGUUGcUGCGGcugucUGCGCGgGAc-- -3' miRNA: 3'- -GGUACAACuACGCC-----AUGCGCgCUucc -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 79715 | 0.72 | 0.884234 |
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Target: 5'- --uUGgcGAUGUGGUACGUGCGcAGa -3' miRNA: 3'- gguACaaCUACGCCAUGCGCGCuUCc -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 84234 | 0.72 | 0.869471 |
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Target: 5'- gCCAcUGUUGA-GCGGcaaACGCGCGccGGc -3' miRNA: 3'- -GGU-ACAACUaCGCCa--UGCGCGCuuCC- -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 18143 | 0.73 | 0.836442 |
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Target: 5'- gCCAcUGUgaguuacUGGUGCGGcGCGCGCGAc-- -3' miRNA: 3'- -GGU-ACA-------ACUACGCCaUGCGCGCUucc -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 70027 | 0.75 | 0.754197 |
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Target: 5'- gCCGUGUUGGcauugauugUGCGGU-CGCuGCGAcGGa -3' miRNA: 3'- -GGUACAACU---------ACGCCAuGCG-CGCUuCC- -5' |
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| 6770 | 5' | -50.9 | NC_001875.2 | + | 90549 | 1.14 | 0.004429 |
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Target: 5'- uCCAUGUUGAUGCGGUACGCGCGAAGGg -3' miRNA: 3'- -GGUACAACUACGCCAUGCGCGCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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