Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6783 | 5' | -49.9 | NC_001875.2 | + | 39657 | 0.7 | 0.916978 |
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Target: 5'- gCGCGAguuuGGGCCGGCGGCGc-------- -3' miRNA: 3'- -GCGUU----UCCGGUCGCCGUaacaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 39168 | 0.71 | 0.890296 |
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Target: 5'- cCGCGAggcGGGCCGGCGGUugcgUGgcgUUUAc -3' miRNA: 3'- -GCGUU---UCCGGUCGCCGua--ACaa-AAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 38310 | 0.71 | 0.882969 |
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Target: 5'- aGCGccgacAAGGCCGGCGGCAUg------- -3' miRNA: 3'- gCGU-----UUCCGGUCGCCGUAacaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 36341 | 0.7 | 0.928702 |
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Target: 5'- gCGCGuguacgcGGCCGGCGGCGacuUUGUcgUCu -3' miRNA: 3'- -GCGUuu-----CCGGUCGCCGU---AACAaaAGu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 26467 | 0.7 | 0.928702 |
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Target: 5'- gCGCAAAacGGCCucGCGGuCGUaGUUUUCGc -3' miRNA: 3'- -GCGUUU--CCGGu-CGCC-GUAaCAAAAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 22590 | 0.67 | 0.983796 |
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Target: 5'- gCGCGuuGGCCGGCcGGC--UGUacggUUUCGu -3' miRNA: 3'- -GCGUuuCCGGUCG-CCGuaACA----AAAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 18621 | 0.66 | 0.988964 |
|
Target: 5'- aGCAAAGGCgCAGCucGGU--UGUUgugUCGu -3' miRNA: 3'- gCGUUUCCG-GUCG--CCGuaACAAa--AGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 6260 | 0.69 | 0.954956 |
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Target: 5'- aCGCAaacGAGGCC-GUGGCAacguuugcuuuugauUUGUUUUUg -3' miRNA: 3'- -GCGU---UUCCGGuCGCCGU---------------AACAAAAGu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 551 | 0.67 | 0.985689 |
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Target: 5'- uGUGGAuguaGCCGGUGGCGUUGUgcaagucgUUCAa -3' miRNA: 3'- gCGUUUc---CGGUCGCCGUAACAa-------AAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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