Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6786 | 3' | -53.7 | NC_001875.2 | + | 29259 | 0.66 | 0.949496 |
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Target: 5'- uGCGGGUGCGcgGCCGCcGAccgcgccggcGGGGcgcggucgUGUGg -3' miRNA: 3'- -CGUCCGCGUa-UGGCGaCU----------UCCU--------ACAC- -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 10682 | 0.66 | 0.930168 |
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Target: 5'- cGCAGGCGC-UGCCGCaacgGcuGGAc--- -3' miRNA: 3'- -CGUCCGCGuAUGGCGa---CuuCCUacac -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 18537 | 0.67 | 0.90678 |
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Target: 5'- uGCuGGUGUacGUGCUGCUGGGcguGGGUGUc -3' miRNA: 3'- -CGuCCGCG--UAUGGCGACUU---CCUACAc -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 72569 | 0.67 | 0.900303 |
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Target: 5'- cGCGGGCGCGgg-CGCUGc-GGGUGc- -3' miRNA: 3'- -CGUCCGCGUaugGCGACuuCCUACac -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 97397 | 0.68 | 0.886609 |
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Target: 5'- uGCGcGCGCAUGgCGUUGAuGGgcGUGa -3' miRNA: 3'- -CGUcCGCGUAUgGCGACUuCCuaCAC- -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 65436 | 0.68 | 0.8794 |
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Target: 5'- --cGGCGUGcACCGCUGgcGGucgGUGg -3' miRNA: 3'- cguCCGCGUaUGGCGACuuCCua-CAC- -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 6978 | 0.7 | 0.766874 |
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Target: 5'- uGCAgGGCGCAaagcgGCCGgUGu-GGGUGUGc -3' miRNA: 3'- -CGU-CCGCGUa----UGGCgACuuCCUACAC- -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 43323 | 0.71 | 0.716739 |
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Target: 5'- cGCGGcGgGCGUGCCGCUGAu---UGUGu -3' miRNA: 3'- -CGUC-CgCGUAUGGCGACUuccuACAC- -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 62486 | 0.72 | 0.675181 |
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Target: 5'- aGCGGGCGCAcGCCGCUGAc------- -3' miRNA: 3'- -CGUCCGCGUaUGGCGACUuccuacac -5' |
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| 6786 | 3' | -53.7 | NC_001875.2 | + | 75508 | 1.1 | 0.003114 |
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Target: 5'- cGCAGGCGCAUACCGCUGAAGGAUGUGc -3' miRNA: 3'- -CGUCCGCGUAUGGCGACUUCCUACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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