Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 680 | 5' | -46.5 | AC_000018.1 | + | 28054 | 0.67 | 0.964322 |
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Target: 5'- aGUCCugGauCAGAGgagGAAGGGuCUGGGu -3' miRNA: 3'- -CAGGugCc-GUUUCaa-CUUUUU-GACCC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 867 | 0.67 | 0.946186 |
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Target: 5'- ---aGCGGCAGAGgUGGGAAAaaauugccugauCUGGGa -3' miRNA: 3'- caggUGCCGUUUCaACUUUUU------------GACCC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 7289 | 0.69 | 0.916174 |
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Target: 5'- -cCCugGGCAugaaauuucGGGUgauucUGAAAGGCUGaGGg -3' miRNA: 3'- caGGugCCGU---------UUCA-----ACUUUUUGAC-CC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 12794 | 0.71 | 0.82206 |
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Target: 5'- -aCCACGGgGcacGUUGGcgGGCUGGGu -3' miRNA: 3'- caGGUGCCgUuu-CAACUuuUUGACCC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 26049 | 0.71 | 0.811837 |
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Target: 5'- aGUCa--GGCAGAGgaggagaUGGAAGAUUGGGa -3' miRNA: 3'- -CAGgugCCGUUUCa------ACUUUUUGACCC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 25696 | 0.71 | 0.801385 |
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Target: 5'- cUCCGgGGCAGAGUcUGAAAcuGACaccGGGg -3' miRNA: 3'- cAGGUgCCGUUUCA-ACUUU--UUGa--CCC- -5' |
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| 680 | 5' | -46.5 | AC_000018.1 | + | 9068 | 1.13 | 0.00273 |
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Target: 5'- aGUCCACGGCAAAGUUGAAAAACUGGGa -3' miRNA: 3'- -CAGGUGCCGUUUCAACUUUUUGACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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