Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6803 | 3' | -54.5 | NC_001875.2 | + | 46992 | 0.66 | 0.930001 |
Target: 5'- aUGAGCGCaccaCGuUgGAGGAgCUGCUGGu -3' miRNA: 3'- -AUUCGCGc---GCcAgUUUCUgGACGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 103004 | 0.66 | 0.924551 |
Target: 5'- -uGGCGUGCGG-CAAGuGCgCUGCgGGc -3' miRNA: 3'- auUCGCGCGCCaGUUUcUG-GACGaCC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 26039 | 0.66 | 0.924551 |
Target: 5'- gAAGaCGCuugacGUGGcCAaggAAGACCUGCUGa -3' miRNA: 3'- aUUC-GCG-----CGCCaGU---UUCUGGACGACc -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 42163 | 0.66 | 0.923993 |
Target: 5'- --cGuCGCGCGGUCAAAcguaaacGACgCgcgGUUGGg -3' miRNA: 3'- auuC-GCGCGCCAGUUU-------CUG-Ga--CGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 6880 | 0.66 | 0.922867 |
Target: 5'- -cGGCGaCGCGGUCAaacagagcuuucccGAGACCaacgUGCgcucGGc -3' miRNA: 3'- auUCGC-GCGCCAGU--------------UUCUGG----ACGa---CC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 124916 | 0.66 | 0.918849 |
Target: 5'- -uAGCGCGCGGUCcacgcccucGGCCgagcgcacGUUGGu -3' miRNA: 3'- auUCGCGCGCCAGuuu------CUGGa-------CGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 76453 | 0.66 | 0.918849 |
Target: 5'- gGGGCGCGCuGGcggcccacUCAAuGAgCUGCUGc -3' miRNA: 3'- aUUCGCGCG-CC--------AGUUuCUgGACGACc -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 90823 | 0.66 | 0.918849 |
Target: 5'- -cGGCcaCGCGGUUGgcGACCUGCUuuaGGu -3' miRNA: 3'- auUCGc-GCGCCAGUuuCUGGACGA---CC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 97767 | 0.66 | 0.912896 |
Target: 5'- cAAGCGCGUGGcggCAAcGGACgaGCUa- -3' miRNA: 3'- aUUCGCGCGCCa--GUU-UCUGgaCGAcc -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 72949 | 0.66 | 0.912896 |
Target: 5'- -uAGCGCGCGuUCGc-GGCCUGCa-- -3' miRNA: 3'- auUCGCGCGCcAGUuuCUGGACGacc -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 100038 | 0.66 | 0.912896 |
Target: 5'- --uGCGCGCuGGUUuugcuguuugugGAgcugacGGugCUGCUGGg -3' miRNA: 3'- auuCGCGCG-CCAG------------UU------UCugGACGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 57842 | 0.66 | 0.906691 |
Target: 5'- aUGAGCGCGCGaaUCAAGGc---GCUGGa -3' miRNA: 3'- -AUUCGCGCGCc-AGUUUCuggaCGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 86247 | 0.66 | 0.906691 |
Target: 5'- --uGCGCGCcggaGUC--GGGCCcGCUGGa -3' miRNA: 3'- auuCGCGCGc---CAGuuUCUGGaCGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 50553 | 0.66 | 0.900239 |
Target: 5'- -cGGCGCGCGuGUCuuuugcGGGGCauucguuggUGCUGGa -3' miRNA: 3'- auUCGCGCGC-CAGu-----UUCUGg--------ACGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 100889 | 0.66 | 0.900239 |
Target: 5'- --cGCGCGUGGgcgcCAuggcGAGACCUGUgcGGc -3' miRNA: 3'- auuCGCGCGCCa---GU----UUCUGGACGa-CC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 131513 | 0.67 | 0.893541 |
Target: 5'- -cGGCGCGCGGUgCA---GCg-GCUGGa -3' miRNA: 3'- auUCGCGCGCCA-GUuucUGgaCGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 89268 | 0.67 | 0.893541 |
Target: 5'- --cGCGCGCGuG-CGccGACCUggcgcgGCUGGg -3' miRNA: 3'- auuCGCGCGC-CaGUuuCUGGA------CGACC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 65800 | 0.67 | 0.872015 |
Target: 5'- -cGGCGCGCGGU-----GCCUGCgcGGc -3' miRNA: 3'- auUCGCGCGCCAguuucUGGACGa-CC- -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 125415 | 0.67 | 0.872015 |
Target: 5'- -cGGCGCGCaaauGGUgAAgauGGGCUUGCUGu -3' miRNA: 3'- auUCGCGCG----CCAgUU---UCUGGACGACc -5' |
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6803 | 3' | -54.5 | NC_001875.2 | + | 126488 | 0.67 | 0.864378 |
Target: 5'- -cAGCGgGUGGUCAAuGACCaGCa-- -3' miRNA: 3'- auUCGCgCGCCAGUUuCUGGaCGacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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