Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6811 | 3' | -49.6 | NC_001875.2 | + | 35568 | 0.66 | 0.995917 |
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Target: 5'- aCGGCuUUU--UGGGGCACAgcGAAc- -3' miRNA: 3'- -GCCGuAAAccACCCCGUGUuuCUUuc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 108006 | 0.66 | 0.995917 |
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Target: 5'- aGcGCGUgcUUGGUGGGGUACcuGGu--- -3' miRNA: 3'- gC-CGUA--AACCACCCCGUGuuUCuuuc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 46244 | 0.66 | 0.9944 |
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Target: 5'- aGcGCG-UUGGUGGaGGCGCu-AGAAAa -3' miRNA: 3'- gC-CGUaAACCACC-CCGUGuuUCUUUc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 6316 | 0.66 | 0.993486 |
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Target: 5'- -aGCA--UGGUGGGGCAgCGccGAAAa -3' miRNA: 3'- gcCGUaaACCACCCCGU-GUuuCUUUc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 79827 | 0.68 | 0.9833 |
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Target: 5'- cCGGCA--UGGUGGuGC-CAgcAAGAAGGg -3' miRNA: 3'- -GCCGUaaACCACCcCGuGU--UUCUUUC- -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 14427 | 0.68 | 0.981185 |
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Target: 5'- cCGGCA--UGGgcgGGGGUAC-GGGAAu- -3' miRNA: 3'- -GCCGUaaACCa--CCCCGUGuUUCUUuc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 65383 | 0.81 | 0.421655 |
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Target: 5'- gCGcGCGcgUGGUGGGGCugAAAGAGAa -3' miRNA: 3'- -GC-CGUaaACCACCCCGugUUUCUUUc -5' |
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| 6811 | 3' | -49.6 | NC_001875.2 | + | 58496 | 1.11 | 0.007267 |
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Target: 5'- gCGGCAUUUGGUGGGGCACAAAGAAAGg -3' miRNA: 3'- -GCCGUAAACCACCCCGUGUUUCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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