miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
684 3' -56.1 AC_000018.1 + 27023 0.66 0.513799
Target:  5'- --gGGAGGCGCCUGCugccaaccccauuugGGGCugauaGCUCcACa -3'
miRNA:   3'- auaCCUCCGCGGGCG---------------UUUG-----CGAGaUG- -5'
684 3' -56.1 AC_000018.1 + 5521 0.67 0.456128
Target:  5'- cGUGGAGuGCGaaaCCGUcuGCGCcucCUGCg -3'
miRNA:   3'- aUACCUC-CGCg--GGCGuuUGCGa--GAUG- -5'
684 3' -56.1 AC_000018.1 + 9092 0.67 0.456128
Target:  5'- cAUGGA-GCGCuuGgAAGCGCUggGCg -3'
miRNA:   3'- aUACCUcCGCGggCgUUUGCGAgaUG- -5'
684 3' -56.1 AC_000018.1 + 16037 0.68 0.359451
Target:  5'- gUGUGGuGGCagacGCCCGCGccuAUGCUCg-- -3'
miRNA:   3'- -AUACCuCCG----CGGGCGUu--UGCGAGaug -5'
684 3' -56.1 AC_000018.1 + 16081 0.7 0.300806
Target:  5'- --aGGAGGCGCaUCGCcAGGCGCcacagggCUACu -3'
miRNA:   3'- auaCCUCCGCG-GGCG-UUUGCGa------GAUG- -5'
684 3' -56.1 AC_000018.1 + 8019 0.72 0.212
Target:  5'- aGUGGAaaauugaggccuGGCGUuuGUAccuGGCGCUCUACu -3'
miRNA:   3'- aUACCU------------CCGCGggCGU---UUGCGAGAUG- -5'
684 3' -56.1 AC_000018.1 + 15844 1.07 0.000518
Target:  5'- gUAUGGAGGCGCCCGCAAACGCUCUACc -3'
miRNA:   3'- -AUACCUCCGCGGGCGUUUGCGAGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.