Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
684 | 5' | -53 | AC_000018.1 | + | 17437 | 0.69 | 0.574997 |
Target: 5'- ---aGCGCGaccaUCGCGGUaCAcucUCCGCa -3' miRNA: 3'- gacaCGCGCa---AGCGCCA-GUaa-AGGCG- -5' |
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684 | 5' | -53 | AC_000018.1 | + | 33520 | 0.71 | 0.444915 |
Target: 5'- aUGUGUaaguuGUGUUCGUGGUCuuccuugUUCUGCc -3' miRNA: 3'- gACACG-----CGCAAGCGCCAGua-----AAGGCG- -5' |
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684 | 5' | -53 | AC_000018.1 | + | 15878 | 1.13 | 0.000546 |
Target: 5'- cCUGUGCGCGUUCGCGGUCAUUUCCGCg -3' miRNA: 3'- -GACACGCGCAAGCGCCAGUAAAGGCG- -5' |
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684 | 5' | -53 | AC_000018.1 | + | 23706 | 0.69 | 0.552438 |
Target: 5'- gUGUGCuccCGUUgGCGGUCGcUUgacugauuuccUCCGCg -3' miRNA: 3'- gACACGc--GCAAgCGCCAGU-AA-----------AGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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