Results 41 - 49 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6853 | 5' | -57.9 | NC_001875.2 | + | 74110 | 0.69 | 0.625922 |
Target: 5'- cUACGCGcCGCGC-GaCUuuGCGGUGu -3' miRNA: 3'- cAUGUGCaGCGCGaC-GAggCGCCACu -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 89365 | 0.69 | 0.64631 |
Target: 5'- cGUGCGCGUgCGCGUguUGCUgCGUGGc-- -3' miRNA: 3'- -CAUGUGCA-GCGCG--ACGAgGCGCCacu -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 2944 | 0.7 | 0.595393 |
Target: 5'- -cGCGCGcuggCGCGCuUGCUCggcaGCGGUGu -3' miRNA: 3'- caUGUGCa---GCGCG-ACGAGg---CGCCACu -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 111799 | 0.71 | 0.535231 |
Target: 5'- gGUGCACGcggccgacggCGCcugGCUGCUCCGCG-UGAc -3' miRNA: 3'- -CAUGUGCa---------GCG---CGACGAGGCGCcACU- -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 12433 | 0.71 | 0.515626 |
Target: 5'- -gGC-CGUUGCG-UGCgcgCCGCGGUGGa -3' miRNA: 3'- caUGuGCAGCGCgACGa--GGCGCCACU- -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 110271 | 0.72 | 0.440516 |
Target: 5'- cGUACGCGuccacgcuaauaUCGCGCgGC-CCGCGGUa- -3' miRNA: 3'- -CAUGUGC------------AGCGCGaCGaGGCGCCAcu -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 113090 | 0.73 | 0.39685 |
Target: 5'- -cGCGCGUCaGCGCcuuaGCcgCCGUGGUGAg -3' miRNA: 3'- caUGUGCAG-CGCGa---CGa-GGCGCCACU- -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 4497 | 0.75 | 0.297163 |
Target: 5'- -cACACGUUGCGuCUGCUCCGCuGcgGAa -3' miRNA: 3'- caUGUGCAGCGC-GACGAGGCGcCa-CU- -5' |
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6853 | 5' | -57.9 | NC_001875.2 | + | 21893 | 1.09 | 0.001674 |
Target: 5'- cGUACACGUCGCGCUGCUCCGCGGUGAg -3' miRNA: 3'- -CAUGUGCAGCGCGACGAGGCGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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