Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 6853 | 5' | -57.9 | NC_001875.2 | + | 108989 | 0.66 | 0.810786 |
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Target: 5'- -gACGCaaucaacucgGUCGCGCaaacGCUUCGCGGcGAa -3' miRNA: 3'- caUGUG----------CAGCGCGa---CGAGGCGCCaCU- -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 4901 | 0.66 | 0.810786 |
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Target: 5'- --uCGCGcCGCaGCUGCUCC-CGGUc- -3' miRNA: 3'- cauGUGCaGCG-CGACGAGGcGCCAcu -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 42586 | 0.66 | 0.801976 |
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Target: 5'- -cGCACG-CGCGCgucaGCUCgGCGcUGGg -3' miRNA: 3'- caUGUGCaGCGCGa---CGAGgCGCcACU- -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 64760 | 0.66 | 0.801976 |
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Target: 5'- uUGCGCGcguagaucUCGCGCgccaGCUCCaGCGGcuUGGu -3' miRNA: 3'- cAUGUGC--------AGCGCGa---CGAGG-CGCC--ACU- -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 71919 | 0.66 | 0.816858 |
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Target: 5'- cGUGCGCGUCGgcgagcgccuugacCGCgcGCUCCGCGc--- -3' miRNA: 3'- -CAUGUGCAGC--------------GCGa-CGAGGCGCcacu -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 23783 | 0.66 | 0.814266 |
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Target: 5'- -cGCACGUugcccaggucgccgaCGUGUcGCUCCGCGGc-- -3' miRNA: 3'- caUGUGCA---------------GCGCGaCGAGGCGCCacu -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 42686 | 0.66 | 0.810786 |
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Target: 5'- cGUGCACGaaacCGCGCgGCg-CGCGGUu- -3' miRNA: 3'- -CAUGUGCa---GCGCGaCGagGCGCCAcu -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 6970 | 0.66 | 0.810786 |
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Target: 5'- --cCACGgCGCGCUGCUUgacaGCGGg-- -3' miRNA: 3'- cauGUGCaGCGCGACGAGg---CGCCacu -5' |
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| 6853 | 5' | -57.9 | NC_001875.2 | + | 85835 | 0.66 | 0.819435 |
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Target: 5'- -aGCACGUCGUGCaccGCguugUUGUGGUGc -3' miRNA: 3'- caUGUGCAGCGCGa--CGa---GGCGCCACu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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