Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6872 | 5' | -46.3 | NC_001875.2 | + | 3488 | 1.1 | 0.017822 |
Target: 5'- gAUAAUGUGAUUCGUACGUGACAGGACg -3' miRNA: 3'- -UAUUACACUAAGCAUGCACUGUCCUG- -5' |
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6872 | 5' | -46.3 | NC_001875.2 | + | 104726 | 0.7 | 0.991494 |
Target: 5'- ---cUGUGGcaaUGUGCGUGAaCGGGACg -3' miRNA: 3'- uauuACACUaa-GCAUGCACU-GUCCUG- -5' |
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6872 | 5' | -46.3 | NC_001875.2 | + | 54234 | 0.7 | 0.992655 |
Target: 5'- -cGAUGUacucGAgcUCGUuccACGUGACGGGGCu -3' miRNA: 3'- uaUUACA----CUa-AGCA---UGCACUGUCCUG- -5' |
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6872 | 5' | -46.3 | NC_001875.2 | + | 84677 | 0.69 | 0.9954 |
Target: 5'- ----cGUGcagCGUugGUGGCGGGAa -3' miRNA: 3'- uauuaCACuaaGCAugCACUGUCCUg -5' |
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6872 | 5' | -46.3 | NC_001875.2 | + | 6760 | 0.69 | 0.996103 |
Target: 5'- -cGAUGUGccgcgcAUUCGcgGCGUGuuuCAGGGCa -3' miRNA: 3'- uaUUACAC------UAAGCa-UGCACu--GUCCUG- -5' |
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6872 | 5' | -46.3 | NC_001875.2 | + | 128388 | 0.67 | 0.999454 |
Target: 5'- ----aGUGuugUCuUAuCGUGACAGGACg -3' miRNA: 3'- uauuaCACua-AGcAU-GCACUGUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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